CoChat
Sign Up
Cited by 192Skin and Cellular Biology ResearchHair Growth and DisordersWound Healing and Treatments

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Human Epidermis Reconstructed by Culture: Is It “Normal”?

Open access
Asselineau, Daniel, Bernard, Bruno A, Bailly, Catherine, Darmon, Michel, Pruniéras, Michel·1986·Journal of Investigative Dermatology
DOI 10.1111/1523-1747.ep12284237
Open this paper in CoChat — free account

Abstract

Human keratinocytes were grown on a dermal equivalent (or lattice) at the liquid-air interface in an attempt to reconstitute a functional epidermis in vitro. Although the multilayered epithelium thus obtained is well differentiated, as shown by the presence of keratohyaline granules and horny layer, several differences from its in vivo counterpart were also observed: In the reconstructed epidermis, basal keratinocytes do not have the cuboidal shape found in vivo; they synthesize bullous pemphigoid antigen and laminin, but the distribution of these antigens is not linear as in vivo; they contain the plasma-membrane antigens restricted to the basal layer in vivo (VM1, BC1), but these antigens are not polarized; lack of polarization is also evidenced by the distribution of actin. Differentiation markers appear but with a topography slightly different from that of epidermis in vivo; the 67-kD keratin does not appear in the first suprabasal layer as in vivo but above; involucrin, which appears in the granular layers in vivo appears as soon as the cells leave the basal layer. psi 3 antigen and fibronectin found in vivo only in hyperproliferative epidermis (wound healing, psoriasis) are detected. Hyperproliferation would also explain the unexpected straining of basal cells by KL1 monoclonal antibody. Because of the potential clinical or pharmacologic use of artificial epidermis, the question of whether the epidermis obtained in vitro can be considered as "normal" is discussed.

Overview

Integrity signals

Registry

Crossref · 10.1111/1523-1747.ep12284237

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 34 references

Research works printed but not linked: 3

  1. Pruniéras, 1979, Nouveau procédé de culture des cellules épidermiques humaines sur derme homologue ou hétérologue: préparation de greffons recombinés, Ann Chir Plast, 24, 357
  2. Chamson, 1982, Differentiation and morphogenesis of keratinocytes grown on contracted collagen lattices, J Cell Biol, 95, 59A
  3. Régnier, 1981, Growth and differentiation of adult human epidermal cells on dermal substrates, Frontiers Matrix Biol, 9, 4

Cited by

Sorted by citations

Related

See all

Metadata

Authors
Asselineau, D., Bernard, B. A., Bailly, C., Darmon, M., & Pruniéras, M.
ORCID
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
1986-02
Volume
86
Issue
2
Pages
181-186
ISSN
0022-202X (ISSN-L)
DOI
10.1111/1523-1747.ep12284237
OpenAlex
W2019032943
Cited by
192
References
34
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 3 · 2018: 5 · 2017: 4 · 2016: 3 · 2014: 2 · 2013: 2
FWCI
4.87
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
  • Health Sciences › Medicine › Dermatology › Hair Growth and Disorders
  • Health Sciences › Medicine › Surgery › Wound Healing and Treatments
Keywords
reconstructed epidermis, human keratinocytes, dermal equivalent, keratinocyte differentiation, basal keratinocytes, bullous pemphigoid antigen, laminin, plasma membrane antigens, actin polarization, involucrin, fibronectin, hyperproliferation
MeSH terms
Dystonin, Epidermal Cells, Collagen Type XVII, Antibodies, Monoclonal, Antigens, Autoantigens, Carrier Proteins, Cell Differentiation, Cells, Cultured, Collagen, Cytoskeletal Proteins, Desmosomes, Epidermis, Humans, Keratins, Laminin, Nerve Tissue Proteins, Non-Fibrillar Collagens

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review