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Cited by 280Skin and Cellular Biology ResearchAdvancements in Transdermal Drug DeliveryHair Growth and Disorders

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Terminal Epidermal Differentiation of Human Keratinocytes Grown in Chemically Defined Medium on Inert Filter Substrates at the Air-Liquid Interface

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Rosdy, Martin, Clauss, Louis-Christian·1990·Journal of Investigative Dermatology
DOI 10.1111/1523-1747.ep12555510
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Abstract

A fully differentiated epithelium having the features of epidermis was obtained in vitro by culturing second-passage normal human keratinocytes (NHK) in the chemically defined medium MCDB 153 on inert filter substrates at the air-liquid interface for 14 d. Vertical sections stained for histology and indirect immunofluorescence studies show a correct stratification and expression of differentiation markers. The presence of desmosomes, keratohyalin granules, and lamellar granules, and the formation of a more than ten-layers stratum corneum was evidenced by electron microscopy. Moreover, lipids typical for differentiated epidermis were present in these cultures, including ceramides, which are thought to be responsible for the relative impermeability of the stratum corneum. Under our culture conditions, i.e., in defined medium and at the air-liquid interface, the use of de-epidermized dermis as a substrate did not stimulate keratinocyte differentiation more than acetate cellulose or polycarbonate filter membrane substrates. The obtaining of a well-differentiated epidermis grown in vitro on inert filters in a chemically defined medium should be useful as a standard system for studying epidermal differentiation, re-epidermization, cytotoxicity, epidermal permeation, and transepidermal drug delivery.

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Research works printed but not linked: 31

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Metadata

Authors
Rosdy, M., & Clauss, L.-C.
Affiliations
  • Université Côte d'Azur (FR)
  • Corning (France) (FR)
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
1990-10
Volume
95
Issue
4
Pages
409-414
ISSN
0022-202X (ISSN-L)
DOI
10.1111/1523-1747.ep12555510
OpenAlex
W1990896467
Cited by
280
References
1
Citations by year
2026: 7 · 2025: 3 · 2024: 8 · 2023: 5 · 2022: 9 · 2021: 15 · 2020: 7 · 2019: 10 · 2018: 13 · 2017: 8 · 2016: 10 · 2015: 11 · 2014: 9 · 2013: 6 · 2012: 9
FWCI
0.86
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
  • Health Sciences › Medicine › Dermatology › Hair Growth and Disorders
Keywords
keratinocyte differentiation, epidermal differentiation, air-liquid interface culture, chemically defined medium, stratum corneum, desmosomes, keratohyalin granules, lamellar granules, ceramide, re-epithelialization, cytotoxicity, epidermal permeability barrier
MeSH terms
Epidermal Cells, Filaggrin Proteins, Adult, Air, Antibodies, Monoclonal, Cell Differentiation, Cells, Cultured, Culture Media, Epidermis, Fluorescent Antibody Technique, Humans, Intermediate Filament Proteins, Keratins, Microscopy, Electron, Protein Precursors, Transglutaminases, Keratinocytes

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