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Cited by 50Immunotherapy and Immune ResponsesT-cell and B-cell ImmunologyAdvancements in Transdermal Drug Delivery

Comparison of Effects of Transforming Growth Factor-Beta and Cyclosporin A on Antigen-Presenting Cells of Blood and Epidermis

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Demidem, Aicha, Taylor, J. Richard, Grammer, Susan F., Streilein, J. Wayne·1991·Journal of Investigative Dermatology
DOI 10.1111/1523-1747.ep12469761
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Abstract

The antigen-processing and -presenting functions of freshly obtained epidermal Langerhans cells (fresh LC) and 72-h cultured Langerhans cells (cultured LC) differ remarkably. It has been proposed that the disparate functional programs revealed in vitro may correspond directly with distinct in vivo physiologic functions--fresh LC are the in vitro equivalent of intraepidermal LC and cultured LC are equivalent to LC that have migrated from skin to the draining lymph node. As an approach to studying this proposal, we have compared the effects of two immunosuppressive agents, cyclosporin A (CsA) and transforming growth factor-beta (TGF beta), on the alloantigen-presenting capabilities of fresh LC, cultured LC, and peripheral blood mononuclear cells (PBMC). CsA pretreatment (1 and 10 mu/ml x 2 h) profoundly inhibited alloantigen presentation by fresh LC, cultured LC, and PBMC. By contrast, TGF beta pretreatment (1 and 10 ng/ml x 2 h) inhibited presentation by PBMC and cultured LC, but not by fresh LC. The resistance of fresh LC to the deleterious effects of TGF beta is discussed in terms of the possibility that TGF beta may inhibit antigen processing following conventional endocytosis. We suggest that fresh, but not cultured, LC escape TGF beta effects because they possess an "alternative" endocytic pathway, marked by the presence of Birbeck granules.

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  • TGF-β lowers reactive oxygen species and nitric oxide, which macrophages need for their destructive functions.

    “TGF-β also suppresses reactive oxygen species and NO production, which is required for the destructive properties of macrophages178,179.”

    From TGFβ biology in cancer progression and immunotherapy (2021) · cites this paper as [179]

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Authors
Demidem, A., Taylor, J. R., Grammer, S. F., & Streilein, J. W.
Affiliations
University of Miami (US)
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
1991-04
Volume
96
Issue
4
Pages
401-407
ISSN
0022-202X (ISSN-L)
DOI
10.1111/1523-1747.ep12469761
OpenAlex
W2083155488
Cited by
50
References
45
Citations by year
2021: 1 · 2020: 1
FWCI
3.82
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (7r01ai022072-08)
  • National Institute of Allergy and Infectious Diseases (AI 22072)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
Keywords
Langerhans cells, antigen presentation, TGF-beta, cyclosporine, immunosuppression, epidermis, peripheral blood mononuclear cells, alloantigen, antigen processing, endocytosis, Birbeck granules, skin-draining lymph nodes
MeSH terms
Epidermal Cells, Antigen-Presenting Cells, Blood Cells, Cyclosporins, Humans, Langerhans Cells, Lymphocyte Culture Test, Mixed, T-Lymphocytes, Transforming Growth Factor beta

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