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Mast‐cell heterogeneity: functional comparison of purified mouse cutaneous and peritoneal mast cells

He, Dan, Esquenazi-Behar, Susana, Soter, Nicholas A, Lim, Henry W·1990·Journal of Investigative Dermatology
DOI 10.1111/1523-1747.ep12477951
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Abstract

To investigate the functional heterogeneity of mouse mast cells, we extracted and purified cutaneous and peritoneal mast cells from 10- to 18-week-old BALB/c mice and compared their responses to secretagogues. Cutaneous mast cells (CMC) were extracted from mouse ears after digestion with hyaluronidase and collagenase in MEM containing 25% fetal calf serum and purified on a discontinuous Percoll gradient. The histamine content of cells obtained from the 30/40% interface was 1.0 +/- 0.1 pg/cell (mean +/- SE), with a mast-cell purity of 68.6 +/- 4.4% and a viability of greater than 93%. Peritoneal mast cells (PMC) were obtained by lavage with modified Tyrode's buffer followed by purification on 22.5% and 3-9% metrizamide gradients. The histamine content of cells was 12.2 +/- 0.8 pg/cell, with a mast-cell purity of 95.9 +/- 0.6% and a viability of greater than 95%. Histamine release induced by A23187 from CMC peaked at 3.0 microM A23187 (19.1 +/- 4.2%), at 3.0 min (22.3 +/- 2.3%), and at 30 degrees C (17.6 +/- 2.6%). In contrast, histamine release from PMC peaked at 8.0 microM of A23187 (49.4 +/- 12.1%) and at 15.0 min (48.5 +/- 12.2%). Release of histamine from PMC was observed at all the temperatures tested from 22 to 45 degrees C. Histamine release from CMC and PMC induced by A23187 was calcium dependent. Histamine release induced by compound 48/80 from CMC peaked at 0.5 micrograms/ml of compound 48/80 (23.0 +/- 7.4%) and at 5.0 min incubation (16.3 +/- 2.0%), whereas release…

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Research works printed but not linked: 28

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  3. Enerback, 1987, Mucosal mast cells in the rat and in man, Int Arch Allergy Appl Immunol, 82, 249, 10.1159/000234199 · DOI
  4. Befus, 1982, Mucosal mast cells. I. Isolation and functional characteristics of rat intestinal mast cells, J Immunol, 128, 2475, 10.4049/jimmunol.128.6.2475 · DOI
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Metadata

Authors
He, D., Esquenazi-Behar, S., Soter, N. A., & Lim, H. W.
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
1990-08
Volume
95
Issue
2
Pages
178-185
ISSN
0022-202X
DOI
10.1111/1523-1747.ep12477951
Cited by
16
References
0
Language
en
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CoChat. (2026). Citation check: Mast‐cell heterogeneity: functional comparison of purified mouse cutaneous and peritoneal mast cells. https://app.cochat.ai/papers/10.1111/1523-1747.ep12477951
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He, D., Esquenazi-Behar, S., Soter, N. A., & Lim, H. W. (1990). Mast‐cell heterogeneity: functional comparison of purified mouse cutaneous and peritoneal mast cells. Journal of Investigative Dermatology. https://doi.org/10.1111/1523-1747.ep12477951