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Cited by 192Skin and Cellular Biology ResearchHair Growth and DisordersWound Healing and Treatments

Human Epidermis Reconstructed by Culture: Is It “Normal”?

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Asselineau, Daniel, Bernard, Bruno A, Bailly, Catherine, Darmon, Michel, Pruniéras, Michel·1986·Journal of Investigative Dermatology
DOI 10.1111/1523-1747.ep12284237
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Abstract

Human keratinocytes were grown on a dermal equivalent (or lattice) at the liquid-air interface in an attempt to reconstitute a functional epidermis in vitro. Although the multilayered epithelium thus obtained is well differentiated, as shown by the presence of keratohyaline granules and horny layer, several differences from its in vivo counterpart were also observed: In the reconstructed epidermis, basal keratinocytes do not have the cuboidal shape found in vivo; they synthesize bullous pemphigoid antigen and laminin, but the distribution of these antigens is not linear as in vivo; they contain the plasma-membrane antigens restricted to the basal layer in vivo (VM1, BC1), but these antigens are not polarized; lack of polarization is also evidenced by the distribution of actin. Differentiation markers appear but with a topography slightly different from that of epidermis in vivo; the 67-kD keratin does not appear in the first suprabasal layer as in vivo but above; involucrin, which appears in the granular layers in vivo appears as soon as the cells leave the basal layer. psi 3 antigen and fibronectin found in vivo only in hyperproliferative epidermis (wound healing, psoriasis) are detected. Hyperproliferation would also explain the unexpected straining of basal cells by KL1 monoclonal antibody. Because of the potential clinical or pharmacologic use of artificial epidermis, the question of whether the epidermis obtained in vitro can be considered as "normal" is discussed.

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  • ALI culture closely mimics the physiological complexity of human skin tissue, including its multilayered structure comprising the epidermis, dermis, and hypodermis.

    “Thorough characterization of skin OTC models is imperative for precise modeling utilizing the ALI culture method, which closely mimics the physiological complexity of human skin tissue, including its multilayered structure that comprises three distinct layers: the epidermis, dermis, and the innermost hypodermis [9,24].”

    From Diversity of human skin three-dimensional organotypic cultures (2024) · cites this paper as [24]

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Authors
Asselineau, D., Bernard, B. A., Bailly, C., Darmon, M., & Pruniéras, M.
ORCID
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
1986-02
Volume
86
Issue
2
Pages
181-186
ISSN
0022-202X (ISSN-L)
DOI
10.1111/1523-1747.ep12284237
OpenAlex
W2019032943
Cited by
192
References
34
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 3 · 2018: 5 · 2017: 4 · 2016: 3 · 2014: 2 · 2013: 2
FWCI
4.87
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
Keywords
reconstructed epidermis, human keratinocytes, dermal equivalent, keratinocyte differentiation, basal keratinocytes, bullous pemphigoid antigen, laminin, plasma membrane antigens, actin polarization, involucrin, fibronectin, hyperproliferation
MeSH terms
Dystonin, Epidermal Cells, Collagen Type XVII, Antibodies, Monoclonal, Antigens, Autoantigens, Carrier Proteins, Cell Differentiation, Cells, Cultured, Collagen, Cytoskeletal Proteins, Desmosomes, Epidermis, Humans, Keratins, Laminin, Nerve Tissue Proteins, Non-Fibrillar Collagens

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