De novo generation of a phosphate starvation-regulated promoter in Candida glabrata
Open accessAbstract
What steps are required for a promoter to acquire regulation by an environmental condition? We address this question by examining a promoter in Candida glabrata that is regulated by phosphate starvation and the transcription factor Pho4. The gene PMU2 encodes a secreted acid phosphatase that resulted from gene duplication events not present in other Ascomycetes, and only this gene of the three paralogs has acquired Pho4 regulation. We observe that the PMU2 promoter from C. glabrata is not functional in Saccharomyces cerevisiae, which is surprising because it is regulated by Pho4, and Pho4 is regulated in a similar manner in both species - through phosphorylation and localization. Additionally, we determine that phosphate starvation-regulated promoters in C. glabrata do not require the coactivator Pho2, which is essential to the phosphate starvation response in S. cerevisiae. We define a region of the PMU2 promoter that is important for Pho4 regulation, and this promoter region does not contain the canonical CACGTX sequence that ScPho4 utilizes for phosphate starvation-dependent transcription. However, CgPho4 utilizes CACGTX in the CgPHO84 promoter, as mutation of this sequence decreases transcription. We conclude that the acquisition of PMU2 has expanded the binding specificity of CgPho4 relative to ScPho4.
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CgPMU2 and CgPHO gene expression is regulated by CgPho4 in a largely CgPho2 coactivator-independent manner.
“Furthermore, CgPMU2 and CgPHO gene expression was found to be regulated by CgPho4 in a largely CgPho2 coactivator-independent manner [115,117].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [117]
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Candida glabrata: A Lot More Than Meets the Eye
Kumar, K., Askari, F., Sahu, M. S., & Kaur, R. (2019) · Microorganisms · doi:10.3390/microorganisms7020039 · Cited by 128 · see citing works
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Nahas, J. V., Iosue, C. L., Shaik, N. F., Selhorst, K., He, B. Z., & Wykoff, D. D. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.200303 · Cited by 8 · see citing works
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- Authors
- Kerwin, C. L., & Wykoff, D. D.
- Affiliations
- Villanova University (US)
- ORCID
- Wykoff, Dennis D.
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-10-02
- Volume
- 12
- Issue
- 8
- Pages
- 980-989
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W2139567947
- Cited by
- 9
- References
- 29
- Citations by year
- 2026: 1 · 2023: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 2 · 2014: 2
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Yeast Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0747799, 1121714)
- Villanova University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- phosphate limitation, Candida glabrata, Saccharomyces cerevisiae, transcriptional regulation, gene duplication, acid phosphatase, promoter evolution
- MeSH terms
- Acid Phosphatase, Chromatin, DNA-Binding Proteins, DNA, Fungal, Mutation, Phosphates, Phosphorylation, Promoter Regions, Genetic, Transcription Factors, Gene Expression Regulation, Fungal, Sequence Analysis, DNA, Candida glabrata, Immunoprecipitation
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