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Cited by 3Effects of Environmental Stressors on LivestockThermoregulation and physiological responsesGenetic and phenotypic traits in livestock

Comparative analysis of heat stress response in Holstein Friesian and Sahiwal cattle through HSP70 gene expression and promoter DNA methylation

Fariha, Shabbir, Muhammad Shakeel, Anwar, Saima, Azmat, Hamda, Majeed, Muhammad Bilal Bin, Kaul, Haiba·2025·Animal Genetics
DOI 10.1111/age.70048
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Abstract

This study investigated the heat stress response in Holstein Friesian and Sahiwal cattle by examining DNA methylation markers in the HSP70 gene promoter. Heat stress parameters such as temperature-humidity index (THI), average rectal temperature (ART), and heat tolerance coefficient (HTC) were analyzed alongside mRNA production during summer and winter. Blood samples were collected from both Holstein Friesian and Sahiwal cattle breeds to assess heat stress parameters, including THI, ART, and HTC. Seasonal variations in these heat stress parameters were evaluated along with mRNA expression levels. The DNA methylation pattern in the HSP70 gene promoter was assessed using the AvaI enzyme while gene expression was analyzed via quantitative PCR with PPIA as a control. Significant correlations were found between seasons, breed-specific heat stress parameters, HSP70 expression, and DNA methylation. Both Sahiwal and Holstein Friesian cattle exhibited higher HTC when DNA methylation was absent, with Sahiwal cattle consistently demonstrating greater heat tolerance than Holstein Friesians. This study highlights the complex interplay between seasonal changes, breed-specific adaptations, and epigenetic modifications in cattle's heat stress responses. The upregulated HSP70 expression suggests a role in thermotolerance. However, the study focused on a single DNA methylation change; broader investigations into additional epigenetic modifications are needed to fully understand heat stress resilience mechanisms across cattle populations.

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Crossref · 10.1111/age.70048

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  • HSP70 expression was higher in Sahiwal than in Holstein Friesian, suggesting that zebu cattle may have greater heat tolerance.

    “HSP70 expression was higher in Sahiwal than in Holstein Friesian [115], suggesting that zebu cattle may have greater heat tolerance.”

    From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [115]

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Authors
Fariha, Shabbir, M. S., Anwar, S., Azmat, H., Majeed, M. B. B., & Kaul, H.
Affiliations
  • University of Veterinary and Animal Sciences (PK)
  • University of Engineering and Technology Lahore (PK)
Corresponding author
Kaul, Haiba
ORCID
Venue
Animal Genetics
Publisher
Wiley
Published
2025-10
Volume
56
Issue
5
Pages
e70048-e70048
ISSN
  • 0268-9146 (ISSN-L)
  • 1365-2052
DOI
10.1111/age.70048
OpenAlex
W4414985675
Cited by
3
References
37
Citations by year
2026: 3
FWCI
1.84
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 8 d)
Locations
  • Animal Genetics (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Effects of Environmental Stressors on Livestock
  • Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
Keywords
heat stress, HSP70, DNA methylation, promoter methylation, Holstein cows, Sahiwal cattle, heat tolerance, temperature-humidity index, heat tolerance coefficient, seasonal variation, epigenetic regulation, breed differences
MeSH terms
Thermotolerance, Animals, Cattle, Promoter Regions, Genetic, Seasons, HSP70 Heat-Shock Proteins, Heat-Shock Response, DNA Methylation

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