Cited by 53Endometriosis Research and TreatmentPlant and fungal interactionsEctopic Pregnancy Diagnosis and Management

Neutrophil depletion reduces endometriotic lesion formation in mice

Takamura, Masashi, Koga, Kaori, Izumi, Gentaro, Urata, Yoko, Nagai, Miwako, Hasegawa, Akiko, Harada, Miyuki, Hirata, Tetsuya +4 more·2016·American Journal of Reproductive Immunology

Abstract

Problem: Neutrophils are known to be accumulated in endometriosis; however, direct evidence of the impact of neutrophils in the development of endometriosis was lacking. To clarify the importance of neutrophils, we examined the effect of neutrophil depletion on the development of endometriosis. Method of study: Ovariectomized, estradiol‐replaced, 8‐week‐old, female BALB/c mice were injected with endometrial fragments (Day 0). Neutrophils were depleted by anti‐Gr‐1 antibody, either in the early stage (from Day 1 to Day 3, group E) or late stage (from Day 8 to Day 12, group L). Control mice (group C) did not receive antibodies. On Day 14, mice were killed and the number and weight of endometriotic lesions were counted and weighed. Results: The number of endometriotic lesions was significantly less in group E in comparison with Group C and Group L. Weight per lesion did not differ between groups. Conclusion: Neutrophils are essential for the initial formation of endometriosis.

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Crossref · 10.1111/aji.12540

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Authors
Takamura, M., Koga, K., Izumi, G., Urata, Y., Nagai, M., Hasegawa, A., Harada, M., Hirata, T., Hirota, Y., Wada‐Hiraike, O., Fujii, T., & Osuga, Y.
Affiliations
The University of Tokyo (JP)
Corresponding author
Koga, Kaori
Venue
American Journal of Reproductive Immunology
Publisher
Wiley
Published
2016-07-19
Volume
76
Issue
3
Pages
193-198
ISSN
  • 0271-7352 (ISSN-L)
  • 1046-7408
  • 1600-0897
OpenAlex
W2498683695
Cited by
53
References
41
Citations by year
2026: 2 · 2025: 9 · 2024: 7 · 2023: 11 · 2022: 3 · 2021: 6 · 2020: 7 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 1 · 2014: 1
FWCI
3.10
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Reproductive Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Health, Labour and Welfare
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
Keywords
endometriosis, neutrophils, neutrophil depletion, neutrophil recruitment
MeSH terms
Animals, Antibodies, Endometriosis, Female, Mice, Inbred BALB C, Neutrophils, Mice

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