Cited by 101Mesenchymal stem cell researchBone Metabolism and DiseasesPeriodontal Regeneration and Treatments

Insulin‐like growth factor 1 can promote proliferation and osteogenic differentiation of human dental pulp stem cells via m TOR pathway

Feng, Xingmei, Huang, Dan, Lu, Xiaohui, Feng, Guijuan, Xing, Jing, Lu, Jun, Xu, Ke, Xia, Weiwei +4 more·2014·Development, Growth & Differentiation

Abstract

Insulin‐like growth factor 1 (IGF‐1) is a multifunctional peptide that can enhance osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSC s). However, it remains unclear whether IGF‐1 can promote osteogenic differentiation of human dental pulp stem cells (DPSC s). In our study, DPSC s were isolated from the impacted third molars, and treated with IGF‐1. Osteogenic differentiation abilities were investigated. We found that IGF‐1 activated the m TOR signaling pathway during osteogenic differentiation of DPSC s. IGF‐1 also increased the expression of runt‐related transcription factor 2 (RUNX 2), osteocalcin (OCN), osterix (OSX) and collagen type I (COL I) during this process. Rapamycin, an m TOR inhibitor, blocked osteogenic differentiation induced by IGF‐1. Meanwhile, CCK‐8 assay and flow cytometry results demonstrated that 10–200 ng/mL IGF‐1 could enhance proliferation ability of DPSC s and 100 ng/mL was the optimal concentration. In summary, IGF‐1 could promote proliferation and osteogenic differentiation of DPSC s via m TOR pathways, which might have clinical implications for osteoporosis.

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Crossref · 10.1111/dgd.12179

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Authors
Feng, X., Huang, D., Lu, X., Feng, G., Xing, J., Lu, J., Xu, K., Xia, W., Meng, Y., Tao, T., Li, L., & Gu, Z.
Affiliations
  • Affiliated Hospital of Nantong University (CN)
  • Department of Stomatology Affiliated Hospital of Nantong University Nantong China
  • Nantong University (CN)
Corresponding author
Gu, Zhifeng
Venue
Development, Growth & Differentiation
Publisher
Wiley
Published
2014-11-11
Volume
56
Issue
9
Pages
615-624
ISSN
  • 0012-1592 (ISSN-L)
  • 1440-169X
OpenAlex
W2056522693
Cited by
101
References
56
Citations by year
2026: 1 · 2025: 7 · 2024: 7 · 2023: 12 · 2022: 8 · 2021: 10 · 2020: 11 · 2019: 10 · 2018: 7 · 2017: 8 · 2016: 14 · 2015: 6
FWCI
4.68
Type
journal article
Language
en
Open access
closed
Locations
  • Development Growth & Differentiation (publishedVersion)
  • PubMed (publishedVersion)
Funders
Chinese National Natural Science Foundation (81401603)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
Keywords
insulin-like growth factor I, dental pulp stem cells, osteogenic differentiation, mTOR signaling, cell proliferation, Runx2, osteocalcin, Osterix, type I collagen, rapamycin, osteoporosis, third molar
MeSH terms
Adolescent, Adult, Cell Differentiation, Dental Pulp, Female, Humans, Insulin-Like Growth Factor I, Male, Osteogenesis, Stem Cells, Signal Transduction, Cell Proliferation, TOR Serine-Threonine Kinases

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