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Cited by 7Hair Growth and DisordersPluripotent Stem Cells ResearchMesenchymal stem cell research

Generating Skin‐Derived Precursor‐Like Cells From Human‐Induced Pluripotent Stem Cell‐Derived Skin Organoids

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Ahmed, Imaan A., Sun, Jane, Kong, Min Jie, Khosrotehrani, Kiarash, Shafiee, Abbas·2024·Experimental Dermatology
DOI 10.1111/exd.70017
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Abstract

Skin‐derived precursor (SKPs) cells are multipotent stem cells found in the dermis that contribute to wound healing and induce hair follicle neogenesis when transplanted. The clinical application of adult human SKPs, however, is hindered by their loss of potency after in vitro expansion. To overcome this challenge, we aimed to isolate SKPs from human‐induced pluripotent stem cell‐derived skin organoids (SKOs), to enable mass production of these cells for therapeutics. We developed a protocol to isolate skin‐derived precursor‐like cells (SKP‐like cells) from human SKOs. SKP‐like cells derived from SKOs exhibited characteristic spheroid morphology and were capable of self‐renewal in defined SKP growth medium. Immunofluorescence analysis confirmed the expression of key markers, including SOX2, fibronectin and S100β, within the SKP‐like cells. The findings of this pilot study shed light on the potential of SKO‐derived SKP‐like cells for future hair regenerative applications. Furthermore, this research highlights the application of human SKOs as a valuable source for isolating progenitor cells, aiming to advance hair regeneration and restore skin function.

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  • hiPSC-derived skin organoids have hair follicles, pigmentation, dermal stratification, appendage patterning, and innervation.

    “Recent advances in human induced pluripotent stem cell (hiPSC) technology have enabled the generation of skin organoids (SKO) with hair follicles, pigmentation, dermal stratification, appendage patterning, and innervation.[ 25 , 26 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ]”

    From Coordinated Development of Immune Cell Populations in Vascularized Skin Organoids from Human Induced Pluripotent Stem Cells (2025) · cites this paper as [34]

    Figure 1 (Conclusions & Future Perspectives; page not provided) shows hiPSC-derived skin organoids developing hair placodes and hair follicles, supporting the hair-follicle portion of the claim. The copy does not establish pigmentation, dermal stratification, broader appendage patterning, or innervation; searches for those features found no supporting passage. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • Previous human SKO models were devoid of vascularization and immune cells.

    “Previous human SKO models were devoid of vascularization and immune cells.[ 25 , 26 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 59 ]”

    From Coordinated Development of Immune Cell Populations in Vascularized Skin Organoids from Human Induced Pluripotent Stem Cells (2025) · cites this paper as [34]

    The Conclusions & Future Perspectives section says that current skin substitutes lack the complexity of human skin, but does not say that previous SKO models lacked vascularization or immune cells. The Results describe SKO dermis and hair follicles; searches for vascularization, vasculature, blood vessels, and immune cells found no passage addressing the claimed absence. (LLM, conf=0.75)

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Cites

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Research works printed but not linked: 24

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Cited by

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Authors
Ahmed, I. A., Sun, J., Kong, M. J., Khosrotehrani, K., & Shafiee, A.
Affiliations
  • The University of Queensland (AU)
  • Queensland Health (AU)
Corresponding author
  • Khosrotehrani, Kiarash
  • Shafiee, Abbas
ORCID
Venue
Experimental Dermatology
Publisher
Wiley
Published
2024-11
Volume
33
Issue
11
Pages
e70017-e70017
ISSN
  • 0906-6705 (ISSN-L)
  • 1600-0625
DOI
10.1111/exd.70017
OpenAlex
W4404702663
Cited by
7
References
3
Citations by year
2026: 4 · 2025: 3
FWCI
3.31
Type
journal article
Language
en
Open access
Licence
Locations
  • Experimental Dermatology (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
Metro North Hospital and Health Service
Topics
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
skin-derived precursor cells, skin organoids, human induced pluripotent stem cells, cell isolation, self-renewal, SOX2, S100β, hair regrowth, wound healing, hair follicle neogenesis
MeSH terms
Cell Differentiation, Humans, Organoids, Pilot Projects, Skin, Hair Follicle, Multipotent Stem Cells, SOXB1 Transcription Factors, Induced Pluripotent Stem Cells

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