Structural characterisation and antioxidant activity of melanoidins from high‐temperature fermented apple
Abstract
Summary: High‐temperature fermented apple is a new fermented product with characteristic black appearance, which is prepared through natural fermentation of whole fresh apple at controlled high temperature (60–90 °C) and humidity (relative humidity of 50–90%). In this work, melanoidins from high‐temperature fermented apple were obtained by ultrafiltration and subsequent diafiltration. The structural characterisation of melanoidins was investigated in the present study. Results showed that the molecular weight of the obtained melanoidins was about 179 kDa and the melanoidins were comprised primarily of furans by pyrolysis–gas chromatography–mass spectrometry. Antioxidant activities of the high‐temperature fermented apple and its melanoidins were evaluated by ABTS, DPPH, hydroxyl radicals, ferric reducing antioxidant power and Fe 2+ ‐chelating assays. Both high‐temperature fermented apple and its melanoidins showed extremely high antioxidant activities in scavenging hydroxyl radicals. High correlation between the antioxidant activity assayed methods and the physicochemical characteristics of melanoidins was obtained. The contribution of melanoidins to the total antioxidant capacity of high‐temperature fermented apple was determined.
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Fruits vinegar contains bioactive ingredients including polyphenolic acids, organic acids, tetramethylpyrazine, and melanoidins.
“Fruits fermentation produces a bio liquid that contains several functional molecules [4,5] such as organic acids [5,6], polyphenols [7,8], melanoidins [9,10], and tetramethylpyrazine [11,12].”
From Fruits Vinegar: Quality Characteristics, Phytochemistry, and Functionality (2021) · cites this paper as [10]
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- Authors
- Zhu, Z., Zhang, Y., Wang, W., Huang, Z., Wang, J., Li, X., & Sun, S.
- Affiliations
- ZheJiang Academy of Agricultural Sciences (CN)
- Zhejiang University of Technology (CN)
- Corresponding author
- Zhang, Yu
- Venue
- International Journal of Food Science & Technology
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-11-18
- Volume
- 56
- Issue
- 5
- Pages
- 2471-2480
- ISSN
- 0950-5423 (ISSN-L)
- 1365-2621
- OpenAlex
- W3097823920
- Cited by
- 14
- References
- 39
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 1 · 2022: 2 · 2021: 1
- FWCI
- 0.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Food Science & Technology (publishedVersion)
- Funders
- Natural Science Foundation of Zhejiang Province (2018C02048, LGC19B050012)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Potato Plant Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
- Keywords
- melanoidins, spontaneous fermentation, antioxidant activity, hydroxyl radical scavenging, furan derivatives, molecular weight, total antioxidant capacity, ultrafiltration, diafiltration
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