17β‐Estradiol administration promotes delayed cutaneous wound healing in 40‐week ovariectomised female mice

Mukai, Kanae; Nakajima, Yukari; Urai, Tamae; Komatsu, Emi; Nasruddin; Sugama, Junko; Nakatani, Toshio

doi:10.1111/iwj.12336

Cited by 27Wound Healing and TreatmentsEstrogen and related hormone effectsDermatologic Treatments and Research

17β‐Estradiol administration promotes delayed cutaneous wound healing in 40‐week ovariectomised female mice

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Mukai, Kanae, Nakajima, Yukari, Urai, Tamae, Komatsu, Emi, Nasruddin, Sugama, Junko, Nakatani, Toshio·2016·International Wound Journal

DOI 10.1111/iwj.12336PMC PMC7949953

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Abstract

This study investigated the effect of 17β‐estradiol on wound healing in 40‐week ovariectomised female mice. Thirty‐six‐week‐old female mice were divided into three groups: medication with 17β‐estradiol after ovariectomy (OVX + 17β‐estradiol), ovariectomy (OVX) and sham (SHAM). The mice received two full‐thickness wounds, and the OVX + 17β‐estradiol group was administered 17β‐estradiol at 0·01 g/day until healing. In the OVX + 17β‐estradiol group, the ratio of wound area was significantly smaller than those of the OVX and SHAM groups on days 1–3, 5, 6, 8–12 and 9–12, respectively, the numbers of neutrophils and macrophages were significantly smaller than those on days 3 and 7, the ratio of re‐epithelialisation was significantly higher than those on days 3 and 11, the ratio of myofibroblasts was significantly higher than those on day 11 and smaller on day 14, and the ratio of collagen fibres was significantly larger than that of the OVX group on days 7–14. We found that 17β‐estradiol administration promotes cutaneous wound healing in 40‐week female mice by reducing wound area, shortening inflammatory response, and promoting re‐epithelialisation, collagen deposition and wound contraction. Our results suggest that cutaneous wound healing that is delayed because of ageing is promoted by exogenous and continuous 17β‐estradiol administration.

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Topical estrogen application to wounds promotes delayed cutaneous wound healing in 80-week-old female mice
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Self-citation

Authors: Mukai, K., Nakajima, Y., Urai, T., Komatsu, E., Nasruddin, Sugama, J., & Nakatani, T.
Affiliations: Kanazawa University (JP)
Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical, Pharmaceutical and Health Sciences Kanazawa University Kanazawa Japan
Corresponding author: Nakatani, Toshio
ORCID: Mukai, Kanae
Nakajima, Yukari
Komatsu, Emi
Sugama, Junko
Venue: International Wound Journal
Publisher: Wiley
Published: 2014-08-06
Volume: 13
Issue: 5
Pages: 636-644
ISSN: 1742-4801 (ISSN-L)
1742-481X
DOI: 10.1111/iwj.12336
PMC: PMC7949953
OpenAlex: W1932164140
Cited by: 27
References: 23
Citations by year: 2026: 3 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 1
FWCI: 1.94
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: International Wound Journal (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: JSPS KAKENHI (22592363, 25293430)
Kanazawa University
Topics: Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
Keywords: estradiol, skin wound healing, delayed wound healing, ovariectomy, aging, re-epithelialization, collagen deposition, myofibroblasts, wound contraction, inflammation, neutrophils, macrophages, female mice
MeSH terms: Administration, Cutaneous, Animals, Estradiol, Female, Ovariectomy, Skin, Wound Healing, Mice

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17β‐Estradiol administration promotes delayed cutaneous wound healing in 40‐week ovariectomised female mice

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