The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Chemical genomic screening for methylation‐silenced genes in gastric cancer cell lines using 5‐aza‐2′‐deoxycytidine treatment and oligonucleotide microarray
Open accessAbstract
To identify novel methylation‐silenced genes in gastric cancers, we carried out a chemical genomic screening, a genome‐wide search for genes upregulated by treatment with a demethylating agent, 5‐aza‐2′‐deoxycytidine (5‐aza‐dC). After 5‐aza‐dC treatment of a gastric cancer cell line (AGS) 579 genes were upregulated 16‐fold or more, using an oligonucleotide microarray with 39 000 genes. From these genes, we selected 44 known genes on autosomes whose silencing in gastric cancer has not been reported. Thirty‐two of these had CpG islands (CGI) in their putative promoter regions, and all of the CGI were methylated in AGS, giving an estimated number of 421 ± 75 (95% confidence interval) methylation‐silenced genes. Additionally, we analyzed the methylation status of 16 potential tumor‐related genes with promoter CGI that were upregulated four‐fold or more, and 14 of these were methylated in AGS. Methylation status of the 32 randomly selected and 16 potential tumor‐related genes was analyzed in 10 primary gastric cancers, and 42 genes (ABHD9, ADFP, ALDH1A3, ANXA5, AREG, BDNF, BMP7, CAV1, CDH2, CLDN3, CTSL, EEF1A2, F2R, FADS1, FSD1, FST, FYN, GPR54, GREM1, IGFBP3, IGFBP7, IRS2, KISS1, MARK1, MLF1, MSX1, MTSS1, NT5E, PAX6, PLAGL1, PLAU, PPIC, RBP4, RORA, SCRN1, TBX3, TFAP2C, TNFSF9, ULBP2, WIF1, ZNF177 and ZNF559) were methylated in at least one primary gastric cancer. A metastasis suppressor gene, MTSS1, was located in a genomic region with frequent loss of heterozygosity (8q22), and was expressed abundantly in the normal gastric mucosa, suggesting its role in gastric carcinogenesis. (Cancer Sci 2006; 97: 64 –71)
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Global Cancer Statistics, 2002
Parkin, D. M., Bray, F., Ferlay, J., & Pisani, P. (2005) · CA: A Cancer Journal for Clinicians · doi:10.3322/canjclin.55.2.74 · Cited by 18,196
The fundamental role of epigenetic events in cancer
Jones, P. A., & Baylin, S. B. (2002) · Nature Reviews Genetics · doi:10.1038/nrg816 · Cited by 5,648
Aberrant CpG-island methylation has non-random and tumour-type–specific patterns
Costello, J. F., Frühwald, M. C., Smiraglia, D. J., Rush, L. J., Robertson, G. P., Gao, X., Wright, F. A., Feramisco, J. D., Peltomäki, P., Lang, J. C., et al. (2000) · Nature Genetics · doi:10.1038/72785 · Cited by 1,357
Causal Relationship between the Loss of RUNX3 Expression and Gastric Cancer
Li, Q.-L., Ito, K., Sakakura, C., Fukamachi, H., Inoue, K.-I., Chi, X.-Z., Lee, K.-Y., Nomura, S., Lee, C.-W., Han, S.-B., et al. (2002) · Cell · doi:10.1016/s0092-8674(02)00690-6 · Cited by 1,028
Self-citationSuzuki, H., Gabrielson, E., Chen, W., Anbazhagan, R., van Engeland, M., Weijenberg, M. P., Herman, J. G., & Baylin, S. B. (2002) · Nature Genetics · doi:10.1038/ng892 · Cited by 857
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Detection and interpretation of altered methylation patterns in cancer cells
Ushijima, T. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1571 · Cited by 483
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Yamashita, K., Upadhyay, S., Osada, M., Hoque, M. O., Xiao, Y., Mori, M., Sato, F., Meltzer, S. J., & Sidransky, D. (2002) · Cancer Cell · doi:10.1016/s1535-6108(02)00215-5 · Cited by 340
Cited by
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Nguyen, A., Rauch, T. A., Pfeifer, G. P., & Hu, V. W. (2010) · The FASEB Journal · doi:10.1096/fj.10-154484 · Cited by 350
The roles and regulation of TBX3 in development and disease
Khan, S. F., Damerell, V., Omar, R., Du Toit, M., Khan, M., Maranyane, H. M., Mlaza, M., Bleloch, J., Bellis, C., Sahm, B. D. B., et al. (2020) · Gene · doi:10.1016/j.gene.2019.144223 · Cited by 99
“The authors showed that in this gastric cancer cell line, but not in 5 other gastric cancer cell lines tested, methylation of the CpG island in the 5′ region of the TBX3 gene effectively silenced its expression (Yamashita et al., 2006).”
BackgroundDiverse functional networks of Tbx3 in development and disease
Washkowitz, A. J., Gavrilov, S., Begum, S., & Papaioannou, V. E. (2012) · WIREs Systems Biology and Medicine · doi:10.1002/wsbm.1162 · Cited by 58
The T-Box transcription factor 3 in development and cancer
Willmer, T., Cooper, A., Peres, J., Omar, R., & Prince, S. (2017) · BioScience Trends · doi:10.5582/bst.2017.01043 · Cited by 44
Metadata
- Authors
- Yamashita, S., Tsujino, Y., Moriguchi, K., Tatematsu, M., & Ushijima, T.
- Venue
- Cancer Science
- Publisher
- Wiley
- Published
- 2005-12-21
- Volume
- 97
- Issue
- 1
- Pages
- 64-71
- ISSN
- 1347-9032 (ISSN-L)
- 1349-7006
- OpenAlex
- W1969179633
- Cited by
- 253
- References
- 41
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- green
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