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Cited by 82Mechanisms of cancer metastasisCellular Mechanics and InteractionsCancer Cells and Metastasis

Correlation between liver metastasis of the colocalization of actin–related protein 2 and 3 complex and WAVE2 in colorectal carcinoma

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Iwaya, Keiichi, Oikawa, Kosuke, Semba, Seitaro, Tsuchiya, Benio, Mukai, Yasuo, Otsubo, Toshiya, Nagao, Toshitaka, Izumi, Miki +3 more·2007·Cancer Science
DOI 10.1111/j.1349-7006.2007.00488.xPMC PMC11158612
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Abstract

Directed movement of normal cells occurs when actin‐related protein 2 and 3 complex (Arp2/3 complex) triggers the actin polymerization that forms lamellipodia immediately after binding to WAVE2. In order to determine whether the same mechanism correlates with liver metastasis from colorectal cancer, paired mirror sections of 154 cancer specimens (29 cases with liver metastasis and 125 cases without liver metastasis in which T factor, gender, primary tumor site, and age at operation were matched) were examined immunohistochemically for the localization of Arp2 and WAVE2. Expression of both Arp2 and WAVE2 was detected in the same cancer cells in 55 (35.7%) of the 154 cases, but not detected in the normal colonic epithelial cells. Univariate analysis showed that the colocalization was significantly predictive of liver metastasis (risk ratio [RR] 8.760. Likewise, histological grade (RR 2.46), lymphatic invasion (RR 9.95), and tumor budding (RR 4.00) were significant predictors. Among these, colocalization and lymphatic invasion were shown to be independent risk factors by multivariate analysis. Another 59 colorectal specimens were examined for mRNA expression of Arp2 by real time polymerase chain reaction. High mRNA levels of Arp2, that in situ hybridization revealed to be expressed by the cancer cells, were significantly associated with liver metastasis. However, its effect was absorbed by the influence of risk of the colocalization that is closely related to high expression of Arp2. These results indicate that the colocalization of Arp2 and WAVE2 is an independent risk factor for liver metastasis of colorectal carcinoma. (Cancer Sci 2007; 98: 992–999)

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  • Overexpression of the ARP2/3 complex is observed in a variety of human cancers.

    “Overexpression of the ARP2/3 complex has been observed in a variety of human cancers”

    From SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis (2022) · cites this paper as Iwaya, Oikawa et al., 2007

    The Results (Level of Arp2, Arp3, and WAVE mRNA expression; page unavailable) report stronger Arp2 expression in colorectal cancer cells than in adjacent normal epithelial cells. That supports increased expression in colorectal cancer, but not in a variety of cancers. The Discussion (page unavailable) also reports a study finding lower Arp2/3-complex mRNA expression in gastric cancer than in normal gastric tissue. (LLM, conf=0.88)

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Authors
Iwaya, K., Oikawa, K., Semba, S., Tsuchiya, B., Mukai, Y., Otsubo, T., Nagao, T., Izumi, M., Kuroda, M., Domoto, H., & Mukai, K.
Affiliations
  • Tokyo Medical University (JP)
  • Kitasato University (JP)
Corresponding author
Iwaya, Keiichi
ORCID
Venue
Cancer Science
Publisher
Wiley
Published
2007-04-23
Volume
98
Issue
7
Pages
992-999
ISSN
  • 1347-9032 (ISSN-L)
  • 1349-7006
DOI
10.1111/j.1349-7006.2007.00488.x
PMC
PMC11158612
OpenAlex
W1964202526
Cited by
82
References
0
Citations by year
2025: 1 · 2024: 1 · 2023: 7 · 2022: 9 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 5 · 2014: 6 · 2013: 5 · 2012: 8
FWCI
1.99
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Cancer Science (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Japan Society for the Promotion of Science (15590319)
  • Institute of Medical Science, University of Tokyo
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mechanisms of cancer metastasis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Keywords
colorectal cancer, liver metastasis, Arp2/3 complex, Arp2, WAVE2, protein colocalization, actin polymerization, lamellipodia, lymphatic invasion, tumor budding, histological grade, metastasis risk factors, mRNA expression
MeSH terms
Actins, Adenocarcinoma, Female, Humans, Immunohistochemistry, Liver Neoplasms, Male, Neoplasm Invasiveness, RNA, Messenger, Colorectal Neoplasms, Polymerase Chain Reaction, In Situ Hybridization, DNA Primers, Wiskott-Aldrich Syndrome Protein Family, Actin-Related Protein 2, Actin-Related Protein 3

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