The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Increased matrix metalloproteinase-9 (MMP-9) activity observed in chronic wound fluid is related to the clinical severity of the ulcer
Open accessAbstract
BACKGROUND: The pathology of chronic wounds is often characterized by elevated levels of proinflammatory cytokines [e.g. tumour necrosis factor (TNF)-alpha and interleukin (IL)-1beta], proteases [e.g. matrix metalloproteinases (MMPs)] and neutrophil elastase. MMPs specifically have been implicated by a number of studies as the major protease family responsible for the degradation of key factors critical to the ulcer's ability to heal. OBJECTIVES: To assess individual MMPs in chronic wound fluid (CWF) in order to develop improved treatments for chronic ulcers. METHODS: Collagen type I and IV zymography, immunoprecipitation followed by a substrate activity assay, and an indirect enzyme-linked immunosorbent assay were all used to analyse MMP levels in CWF. RESULTS: Our studies demonstrate that there is excessive protease activity in CWF compared with both human serum and acute wound fluid (AWF), which can be specifically attributed to MMPs as determined through a MMP-inhibitor study. Multiple MMPs were immunoprecipitated from the CWF samples and MMP-9 was identified as the predominant protease in CWF, with significantly elevated activity levels in CWF compared with AWF. In addition, the clinical status of the ulcer is directly associated with the amounts of MMP-9 present in the wound fluid. Therefore, this study suggests that higher levels of MMP-9 in chronic wound fluid correlate with a clinically worse wound. CONCLUSIONS: In view of these results, it is hypothesized that a specific inhibitor of MMP-9 could potentially be more therapeutically effective than general MMP inhibitors in modulating chronic ulcers towards a healing state.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Mechanisms involved in the side effects of glucocorticoids
SCHACKE, H. (2002) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(02)00297-8 · Cited by 1,847
Physiology and healing dynamics of chronic cutaneous wounds
Stadelmann, W. K., Digenis, A. G., & Tobin, G. R. (1998) · The American Journal of Surgery · doi:10.1016/s0002-9610(98)00183-4 · Cited by 909
Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors
Trengove, N. J., Stacey, M. C., Macauley, S., Bennett, N., Gibson, J., Burslem, F., Murphy, G., & Schultz, G. (1999) · Wound Repair and Regeneration · doi:10.1046/j.1524-475x.1999.00442.x · Cited by 908
Matrix metalloproteinases: structures, evolution, and diversification
Massova, I., Kotra, L. P., Fridman, R., & Mobashery, S. (1998) · The FASEB Journal · doi:10.1096/fasebj.12.12.1075 · Cited by 781
Wound Fluid from Chronic Leg Ulcers Contains Elevated Levels of Metalloproteinases MMP-2 and MMP-9
Wysocki, A. B., Staiano-Coico, L., & Grinnell, F. (1993) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12359590 · Cited by 732
Cellular Dysfunction in the Diabetic Fibroblast
Lerman, O. Z., Galiano, R. D., Armour, M., Levine, J. P., & Gurtner, G. C. (2003) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)63821-7 · Cited by 504
Okada, Y., Gonoji, Y., Naka, K., Tomita, K., Nakanishi, I., Iwata, K., Yamashita, K., & Hayakawa, T. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)36670-0 · Cited by 501
The proteolytic environment of chronic wounds
Yager, D. R., & Nwomeh, B. C. (1999) · Wound Repair and Regeneration · doi:10.1046/j.1524-475x.1999.00433.x · Cited by 398
Yager, D. R., Zhang, L.-Y., Liang, H.-X., Diegelmann, R. F., & Cohen, I. K. (1996) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12365637 · Cited by 389
Cited by
Neutrophils and Wound Repair: Positive Actions and Negative Reactions
Wilgus, T. A., Roy, S., & McDaniel, J. C. (2013) · Advances in Wound Care · doi:10.1089/wound.2012.0383 · Cited by 616
Dynamic reciprocity in the wound microenvironment
Schultz, G. S., Davidson, J. M., Kirsner, R. S., Bornstein, P., & Herman, I. M. (2011) · Wound Repair and Regeneration · doi:10.1111/j.1524-475x.2011.00673.x · Cited by 490
Proteases and Delayed Wound Healing
McCarty, S. M., & Percival, S. L. (2013) · Advances in Wound Care · doi:10.1089/wound.2012.0370 · Cited by 476
Wound healing in the 21st century
Schreml, S., Szeimies, R.-M., Prantl, L., Landthaler, M., & Babilas, P. (2010) · Journal of the American Academy of Dermatology · doi:10.1016/j.jaad.2009.10.048 · Cited by 416
Defining stem cell dynamics and migration during wound healing in mouse skin epidermis
Aragona, M., Dekoninck, S., Rulands, S., Lenglez, S., Mascré, G., Simons, B. D., & Blanpain, C. (2017) · Nature Communications · doi:10.1038/ncomms14684 · Cited by 384
Metadata
- Authors
- Rayment, E. A., Upton, Z., & Shooter, G. K.
- Venue
- British Journal of Dermatology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-05
- Volume
- 158
- Issue
- 5
- Pages
- 951-961
- ISSN
- 0007-0963 (ISSN-L)
- 1365-2133
- OpenAlex
- W1523254950
- Cited by
- 252
- References
- 35
- FWCI
- 12.95
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rayment, E. A., Upton, Z., & Shooter, G. K. (2008). Increased matrix metalloproteinase-9 (MMP-9) activity observed in chronic wound fluid is related to the clinical severity of the ulcer. British Journal of Dermatology. https://doi.org/10.1111/j.1365-2133.2008.08462.x
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