Modulation of HER2 expression by ferulic acid on human breast cancer MCF7 cells
Abstract
BACKGROUND: The molecular mechanisms underlying the mitogenic effect of ferulic acid (FA), an active compound derived from Angelica sinensis, have never been elucidated. It was the aim of this study to investigate the proliferative effect of FA on human breast cancer cell lines and to elucidate its modulation mechanism on HER2 expression in MCF7 line. MATERIALS AND METHODS: By using MCF7 (oestrogen receptor-positive; ER+, HER2-low), BT474 (ER+, HER2-high), MDAMB231 (ER-, HER2-low) and SKBR3 (ER-, HER2-high) human breast cancer cell lines as in vitro models, the mitogenic effects of FA were assessed by trypan blue dye exclusion assay and DNA flow cytometry. Ferulic acid-modulated cell signalling and HER2 gene expression were evaluated in MCF7 line by Western blot and real-time RT-PCR analysis. RESULTS: Ferulic acid ER-dependently stimulated cell proliferation on MCF7 cells in a concentration-dependent manner. The HER2 oncogene (one of the prognostic factors of breast cancer) and ESR1 gene (oestrogen receptor-alpha; ERalpha) transcription were markedly up-regulated by FA treatment. Besides, HER2 signalling and its downstream molecules such as AKT and ERK1/2 were involved in FA-modulated ERalpha and cyclin D1 synthesis. Addition of anti-HER2 antibody, trastuzumab, abrogated FA-enhanced proliferative effect on MCF7 cells, indicated a positive feedback control for the action of HER2 in this setting. The fact that the ER antagonist blocked most of the FA-up-regulated HER2 expression, and that trastuzumab down-regulated ERalpha gene expression, suggested a cross-talk between ERalpha and HER2 signalling on MCF7 cells. CONCLUSION: The authors' conclude that FA causes human breast cancer cell proliferation by up-regulation of HER2 and ERalpha expression.
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Phenolic acids, in particular ferulic acid, may modulate estrogen receptors or the estrogenic pathway in mammalian cells.
“Moreover, these phenolic acids and in particular ferulic acid may modulate estrogen receptors or the estrogenic pathway in mammalian cells (Chang et al., 2006).”
From RETRACTED: Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize (2012) · cites this paper as Chang et al., 2006
These phenolic acids, and in particular ferulic acid, may modulate estrogen receptors or the estrogenic pathway in mammalian cells.
“Moreover, these phenolic acids, and in particular ferulic acid, may modulate estrogen receptors or the estrogenic pathway in mammalian cells [71].”
From Republished study: long-term toxicity of a Roundup herbicide and a Roundup-tolerantgenetically modified maize (2014) · cites this paper as [71]
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Caffeic and Ferulic Acid Derivatives
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The Need for a Closer Look at Pesticide Toxicity during GMO Assessment
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- Authors
- Chang, C. J., Chiu, J. H., Tseng, L. M., Chang, C. H., Chien, T. M., Wu, C. W., & Lui, W. Y.
- Affiliations
- Taipei Veterans General Hospital (TW)
- United States Department of Veterans Affairs (US)
- National Yang Ming University (TW)
- Corresponding author
- Chiu, J. H.
- Venue
- European Journal of Clinical Investigation
- Publisher
- Wiley
- Published
- 2006-07-18
- Volume
- 36
- Issue
- 8
- Pages
- 588-596
- ISSN
- 0014-2972 (ISSN-L)
- 1365-2362
- OpenAlex
- W2094139685
- Cited by
- 80
- References
- 36
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 2 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 7 · 2015: 6 · 2014: 10 · 2013: 7 · 2012: 6
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Clinical Investigation (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Rotary Foundation
- Topics
- Health Sciences › Medicine › Oncology › HER2/EGFR in Cancer Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- ferulic acid, HER2 expression, breast cancer, MCF-7 cells, estrogen receptor alpha, ESR1, cell proliferation, Akt, ERK1/2, cyclin D1, trastuzumab, Angelica sinensis, mitogen response
- MeSH terms
- Antibodies, Neoplasm, Breast Neoplasms, Coumaric Acids, Dose-Response Relationship, Drug, Female, Humans, Mitogens, Mitosis, Signal Transduction, Up-Regulation, Gene Expression Regulation, Neoplastic, Genes, erbB-2, Cyclin D1, Mitogen-Activated Protein Kinase 1, Cell Line, Tumor, Estrogen Receptor alpha, Proto-Oncogene Proteins c-akt
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