The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Chitosan/double‐stranded RNA nanoparticle‐mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito ( Anopheles gambiae )
Abstract
The purpose of this study was to examine whether the expression of two chitin synthase genes, AgCHS1 and AgCHS2 , can be repressed by chitosan/ AgCHS dsRNA‐based nanoparticles through larval feeding in Anopheles gambiae . The AgCHS1 transcript level and chitin content were reduced by 62.8 and 33.8%, respectively, in the larvae fed on chitosan/ AgCHS1 dsRNA nanoparticles compared with those of the control larvae fed on chitosan/GFP dsRNA nanoparticles. Our study suggested for the first time that RNA interference (RNAi) in mosquito larvae is systemic, and demonstrated that the larvae fed on the nanoparticles assembled from AgCHS1 and AgCHS2 dsRNA increased larval susceptibilities to diflubenzuron, and calcofluor white (CF) or dithiothreitol, respectively. These results suggest great potential for using such a nanoparticle‐based RNAi technology for high‐throughput screening of gene functions and for developing novel strategies for pest management.
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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y. (2007) · Nature Biotechnology · doi:10.1038/nbt1352 · Cited by 1,358 · cited 1×
“Recent studies have shown some great potential for using RNAi technologies to control agriculturally important insect pests (Baum et al., 2007; Mao et al., 2007).”
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Cited by
Biosynthesis, Turnover, and Functions of Chitin in Insects
Zhu, K. Y., Merzendorfer, H., Zhang, W., Zhang, J., & Muthukrishnan, S. (2016) · Annual Review of Entomology · doi:10.1146/annurev-ento-010715-023933 · Cited by 435
Self-citationThe cellular basis of chitin synthesis in fungi and insects: Common principles and differences
Merzendorfer, H. (2011) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2011.04.014 · Cited by 320
Yan, S., Ren, B., & Shen, J. (2021) · Insect Science · doi:10.1111/1744-7917.12822 · Cited by 303
Yan, S., Qian, J., Cai, C., Ma, Z., Li, J., Yin, M., Ren, B., & Shen, J. (2020) · Journal of Pest Science · doi:10.1007/s10340-019-01157-x · Cited by 281
Khajuria, C., Ivashuta, S., Wiggins, E., Flagel, L., Moar, W., Pleau, M., Miller, K., Zhang, Y., Ramaseshadri, P., Jiang, C., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0197059 · Cited by 251
Metadata
- Authors
- Zhang, X., Zhang, J., & Zhu, K. Y.
- Venue
- Insect Molecular Biology
- Publisher
- Wiley
- Published
- 2010-07-08
- Volume
- 19
- Issue
- 5
- Pages
- 683-693
- ISSN
- 0962-1075 (ISSN-L)
- 1365-2583
- OpenAlex
- W1954006000
- Cited by
- 474
- References
- 2
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zhang, X., Zhang, J., & Zhu, K. Y. (2010). Chitosan/double‐stranded RNA nanoparticle‐mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito ( Anopheles gambiae ). Insect Molecular Biology. https://doi.org/10.1111/j.1365-2583.2010.01029.x
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