Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte
Open accessAbstract
The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish a population of DvSnf7 dsRNA-resistant western corn rootworm, Diabrotica virgifera virgifera, a major maize insect pest. WCR resistant to ingested DvSnf7 dsRNA had impaired luminal uptake and resistance was not DvSnf7 dsRNA-specific, as indicated by cross resistance to all other dsRNAs tested. No resistance to the Bacillus thuringiensis Cry3Bb1 protein was observed. DvSnf7 dsRNA resistance was inherited recessively, located on a single locus, and autosomal. Together these findings will provide insights for dsRNA deployment for insect pest control.
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Recent studies have demonstrated that the lack of unexpected effects is also true for a combination of Cry proteins and dsRNA.
“Recent studies have demonstrated that this is also true for a combination of Cry proteins and dsRNA (Khajuria et al., 2018;Levine et al., 2015;Ni et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Khajuria et al., 2018
Resistance to dsRNA was not sequence-specific but caused by impaired luminal uptake, indicated by cross resistance to other dsRNAs tested.
“Interestingly, resistance was not sequence-specific but caused by an impaired luminal uptake, indicated by cross resistance to other dsRNAs tested.”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Khajuria et al., 2018
Combining Bt Cry proteins with RNAi has great potential to delay resistance development.
“Combing Bt Cry proteins with RNAi has great potential to delay resistance development (Khajuria et al., 2018;Ni et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Khajuria et al., 2018
Insects can develop resistance to dsRNA.
“A recent study demonstrated that insects can develop resistance to dsRNA (Khajuria et al., 2018).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Khajuria et al., 2018
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Genetically engineered crops help support conservation biological control
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- Authors
- Khajuria, C., Ivashuta, S., Wiggins, E., Flagel, L., Moar, W., Pleau, M., Miller, K., Zhang, Y., Ramaseshadri, P., Jiang, C., Hodge, T., Jensen, P., Chen, M., Gowda, A., McNulty, B., Vazquez, C., Bolognesi, R., Haas, J., Head, G., & Clark, T.
- Affiliations
- Monsanto (United States) (US)
- Corresponding author
- Khajuria, Chitvan
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2018-05-14
- Volume
- 13
- Issue
- 5
- Pages
- e0197059
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC5951553
- OpenAlex
- W2803644409
- Cited by
- 251
- References
- 44
- Citations by year
- 2026: 33 · 2025: 47 · 2024: 36 · 2023: 18 · 2022: 21 · 2021: 36 · 2020: 31 · 2019: 22 · 2018: 7
- FWCI
- 8.53
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Keywords
- western corn rootworm, RNA interference, cross-resistance, recessive inheritance, autosomal loci, resistance management, maize pest control
- MeSH terms
- Animals, Coleoptera, Zea mays, Pest Control, Biological, RNA, Double-Stranded, Animals, Genetically Modified
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