The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Improvement in the assessment of human epididymal function by the use of inhibitors in the assay of α‐glucosidase in seminal plasma
Open accessAbstract
Seminal alpha-glucosidase has been used clinically as a marker of epididymal function. In this study enzyme inhibitors were used to increase the specificity of the human seminal alpha-glucosidase assay and improve its diagnostic value as an indicator of distal epididymal occlusion in cases of azoospermia. Sodium dodecylsulphate was added to the sample to eliminate the interfering acid isoenzyme secreted by the prostate gland, and castanospermine was used with semen pools to provide a semen blank for the assay by eliminating non-glucosidase-regulated degradation of the substrate. With both inhibitors included in the assay, glucosidase activity in semen samples from 17 fathers was measured to provide reference values for the clinic (lower threshold 18 mU per ejaculate). With the improved assay glucosidase was non-detectable in 8 out of 11 cases of proven and 5 out of 8 cases of suspected ductal obstruction; other azoospermic patients with distal occlusion had values below 11 mU per ejaculate.
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GII activity is significantly reduced when an inflammatory occlusion occurs distally to the epididymis.
“In fact, GII activity is significantly reduced when an inflammatory occlusion distally to the epididymis occurs [440,441,442]”
From GANAB and N-Glycans Substrates Are Relevant in Human Physiology, Polycystic Pathology and Multiple Sclerosis: A Review (2022) · cites this paper as [440]
The Results section (‘Neutral α-glucosidase activity in semen from men with occluded ducts’; page unavailable) reports that the modified assay detected no activity in 8 of 10 men with proven distal ductal occlusion and low activity in the other two. The paper does not identify those occlusions as inflammatory or explicitly identify the measured enzyme as GII, so it does not establish the claim under that specific condition. (LLM, conf=0.86)
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Citations
Cites
Chemistry and Biochemistry of Microbial α‐Glucosidase Inhibitors
Truscheit, E., Frommer, W., Junge, B., Müller, L., Schmidt, D. D., & Wingender, W. (1981) · Angewandte Chemie International Edition in English · doi:10.1002/anie.198107441 · Cited by 473
Castanospermine, a tetrahydroxylated alkaloid that inhibits β-glucosidase and β-glucocerebrosidase
Saul, R., Chambers, J. P., Molyneux, R. J., & Elbein, A. D. (1983) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(83)90181-9 · Cited by 298
Chapdelaine, P., Tremblay, R. R., & Dubé, J. Y. (1978) · Clinical Chemistry · doi:10.1093/clinchem/24.2.208 · Cited by 156
Castanospermine inhibits alpha-glucosidase activities and alters glycogen distribution in animals.
Saul, R., Ghidoni, J. J., Molyneux, R. J., & Elbein, A. D. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.1.93 · Cited by 138
Inhibition of mammalian digestive disaccharidases by polyhydroxy alkaloids
Scofield, A. M., Fellows, L. E., Nash, R. J., & Fleet, G. W. J. (1986) · Life Sciences · doi:10.1016/0024-3205(86)90046-9 · Cited by 137
Epididymal Markers in Human Infertility
Cooper, T. G., HING‐HEIYEUNG, C., Nashan, D., et al. (1988) · Journal of Andrology · doi:10.1002/j.1939-4640.1988.tb01016.x · Cited by 114
Self-citation6-Epicastanospermine, a novel indolizidine alkaloid that inhibits α-glucosidase
Molyneux, R. J., Roitman, J. N., Dunnheim, G., Szumiło, T., & Elbein, A. D. (1986) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(86)90351-6 · Cited by 94
Guérin, J.-F., Ali, H. B., ROLLET, J., et al. (1986) · Journal of Andrology · doi:10.1002/j.1939-4640.1986.tb00901.x · Cited by 67
Knuth, U. A., Yeung, C., & Nieschlag, E. (1989) · Fertility and Sterility · doi:10.1016/s0015-0282(16)60735-5 · Cited by 63
Self-citationYeung, C., Cooper, T. G., & Senge, T. (1990) · Biology of Reproduction · doi:10.1095/biolreprod42.4.669 · Cited by 39
Self-citationCASANO, R., Orlando, C., Caldini, A. L., et al. (1987) · Fertility and Sterility · doi:10.1016/s0015-0282(16)50013-2 · Cited by 34
Similar Biochemical Properties of Human Seminal Plasma and Epididymal α‐1,4‐Glucosidase
Paquin, R., Chapdelaine, P., DUBÉ, J. Y., et al. (1984) · Journal of Andrology · doi:10.1002/j.1939-4640.1984.tb00789.x · Cited by 33
Enzymes in the Seminal Plasma from Azoospermic Men: Correlation with the Origin of their Azoospermia
Guérin, J.-F., ROLLET, J., Perrin, P. B., et al. (1981) · Fertility and Sterility · doi:10.1016/s0015-0282(16)45740-7 · Cited by 33
Origin of Maltase and Variations in Infertile Men
Chapdelaine, P., Tremblay, R. R., Dubé, J. Y., et al. (1978) · Archives of Andrology · doi:10.3109/01485017808988319 · Cited by 20
Castanospermine: An apparent tight-binding inhibitor of hepatic lysosomal alpha-glucosidase
Ellmers, B. R., Rhinehart, B. L., & Robinson, K. M. (1987) · Biochemical Pharmacology · doi:10.1016/0006-2952(87)90607-1 · Cited by 18
Rr, T., Chapdelaine, P., Roy, R., et al. (1982) · PubMed · Cited by 14
Neutral α-1,4-glucosidase in human seminal plasma: molecular forms in varicocele and after vasectomy
Tremblay, R. R., Chapdelaine, P., & Dubé, J. Y. (1982) · Fertility and Sterility · doi:10.1016/s0015-0282(16)46517-9 · Cited by 11
Effect of vasectomy on the prostatic function as indicated by seminal maltase activity
Thakur, A. N., SHETH, A. R., RAO, S. S., et al. (1975) · Contraception · doi:10.1016/0010-7824(75)90026-8 · Cited by 10
α, 1–4 Glucosidase Activity in Human Seminal Plasma from Normal, Vasectomized, and Subfertile Men
Rivas, F., Velázquez, A., Díaz, M., et al. (1981) · Archives of Andrology · doi:10.3109/01485018108999325 · Cited by 8
Guérin, J.-F., & Czyba, J. C. (1987) · Springer eBooks · doi:10.1007/978-1-4613-1953-5_25 · Cited by 6
Purification and properties of neutral α-1,4 glucosidase from human seminal plasma
Chapdelaine, P., Tremblay, R. R., & Dube, J. Y. (1979) · Archives of Andrology · doi:10.3109/01485017908985063 · Cited by 5
Flagged first, then by citations
Research works printed but not linked: 2
- Müller L., 1985, Biotechnology, 1
- Tremblay R. R., 1986, Andrology, Male Fertility anc Sterility, 273
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Diabetes-induced hyperglycemia impairs male reproductive function: A systematic review
Maresch, C. C., Stute, D. C., Alves, M. G., Oliveira, P. F., de Kretser, D. M., & Linn, T. (2018) · Human Reproduction Update · doi:10.1093/humupd/dmx033 · Cited by 294
De Jager, C., Farias, P., Barraza‐Villarreal, A., Avila, M. H., Ayotte, P., Dewailly, E., Dombrowski, C., Rousseau, F., Sanchez, V. D., & Bailey, J. L. (2006) · Journal of Andrology · doi:10.2164/jandrol.05121 · Cited by 167
New discriminatory level for glucosidase activity to diagnose epididymal obstruction or dysfunction
Kret, B., Milad, M., & Jeyendran, R. S. (1995) · Archives of Andrology · doi:10.3109/01485019508987850 · Cited by 27
De Masi, R., & Orlando, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23137373 · Cited by 20
Metadata
- Authors
- COOPER, T. G., YEUNG, C. H., NASHAN, D., JOCKENHÖVEL, F., & NIESCHLAG, E.
- Affiliations
- University of Münster (DE)
- Corresponding author
- COOPER, T. G.
- ORCID
- NIESCHLAG, E.
- Venue
- International Journal of Andrology
- Publisher
- Wiley
- Published
- 1990-08
- Volume
- 13
- Issue
- 4
- Pages
- 297-305
- ISSN
- 0105-6263 (ISSN-L)
- 1365-2605
- OpenAlex
- W2165376568
- Cited by
- 83
- References
- 21
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 3 · 2017: 2 · 2015: 1 · 2014: 1 · 2013: 2
- FWCI
- 3.47
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal of Andrology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Bayer
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Urology › Urologic and reproductive health conditions
- Health Sciences › Medicine › Urology › Male Reproductive Health Studies
- Keywords
- epididymal function, azoospermia, enzyme inhibitors, castanospermine, sodium dodecyl sulfate, reference values, ductal obstruction
- MeSH terms
- Alkaloids, alpha-Glucosidases, Epididymis, Family, Humans, Indolizines, Male, Semen, Trisaccharides, Biomarkers, Acarbose, Indolizidines, Glycoside Hydrolase Inhibitors
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