The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Immunoglobulins from Egg Yolk: Isolation and Purification
Abstract
Simple water dilution was employed for the separation of water‐soluble plasma proteins from egg yolk granules. An optimum recovery of immunoglobulin Y (IgY, 93–96%) in water‐soluble fraction was obtained by sixfold water dilution at pH between 5.0–5.2 with incubation time of 6 hr at 4°C. Among the factors studied, pH was found to be the most important factor affecting IgY recovery. Active IgY of high purity with good recovery was obtained by a combination of several techniques including salt precipitation, alcohol precipitation, ultrafiltration, gel filtration and ion exchange chromatography. Salt precipitation, ultrafiltration, and get filtration is the recommended sequence. Over 100 mg of electrophoretically pure IgY was routinely obtained from one egg.
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Cites
The Avian Egg: Chemistry and Biology
Burley, R. W., & Vadehra, D. V. (1989) · Cited by 493
Eggs: Conveniently packaged antibodies. Methods for purification of yolk IgG
Jensenius, J. C., Andersen, I., Hau, J., Crone, M., & Koch, C. (1981) · Journal of Immunological Methods · doi:10.1016/0022-1759(81)90333-1 · Cited by 366
Tacket, C. O., Losonsky, G., Link, H., Hoang, Y., Guesry, P., Hilpert, H., & Levine, M. M. (1988) · New England Journal of Medicine · doi:10.1056/nejm198805123181904 · Cited by 292
ISOLATION AND COMPOSITION OF AVIAN EGG YOLK GRANULES AND THEIR CONSTITUENT α- AND β-LIPOVITELLINS
Burley, R. W., & Cook, W. H. (1961) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o61-136 · Cited by 234
Rapid micro Kjeldahl digestion of cereal grains and other biological materials
Concon, J. M., & Soltess, D. (1973) · Analytical Biochemistry · doi:10.1016/0003-2697(73)90405-3 · Cited by 126
Bovine milk immunoglobulins for passive immunity to infantile rotavirus gastroenteritis
Brüssow, H., Hilpert, H., Walther, I., Sidoti, J., Mietens, C., & Bachmann, P. (1987) · Journal of Clinical Microbiology · doi:10.1128/jcm.25.6.982-986.1987 · Cited by 111
Ebina, T., Sato, A., Umezu, K., Ishida, N., Ohyama, S., Oizumi, A., Aikawa, K., Katagiri, S., Katsushima, N., Imai, A., et al. (1985) · Medical Microbiology and Immunology · doi:10.1007/bf02123694 · Cited by 107
Burley, R. W., & Vadehra, D. V. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90789-9 · Cited by 32
Cited by
Schade, R., Calzado, E. G., Sarmiento, R., Chacana, P. A., Porankiewicz-Asplund, J., & Terzolo, H. R. (2005) · Alternatives to Laboratory Animals · doi:10.1177/026119290503300208 · Cited by 381
Kajkowski, E. M., Lo, C. F., Ning, X., Walker, S., Sofia, H. J., Wang, W., Edris, W., Chanda, P., Wagner, E., Vile, S., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m011161200 · Cited by 63
Torché, A.-M., Le Corre, P., Albina, E., & Le Verge, R. (1999) · Journal of Drug Targeting · doi:10.3109/10611869909085517 · Cited by 50
Metadata
- Authors
- AKITA, E. M., & NAKAI, S.
- Venue
- Journal of Food Science
- Publisher
- Wiley
- Published
- 1992-05
- Volume
- 57
- Issue
- 3
- Pages
- 629-634
- ISSN
- 0022-1147 (ISSN-L)
- 1750-3841
- OpenAlex
- W2004177219
- Cited by
- 301
- References
- 8
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- AKITA, E. M., & NAKAI, S. (1992). Immunoglobulins from Egg Yolk: Isolation and Purification. Journal of Food Science. https://doi.org/10.1111/j.1365-2621.1992.tb08058.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
