PLGA Microspheres Phagocytosis by Pig Alveolar Macrophages: Influence of Polyvinyl alcohol) Concentration, Nature of Loaded-Protein and Copolymer Nature
Abstract
The study aimed to investigate on a pig alveolar macrophage culture model the influence of: (1) poly(D,L-lactide-co-glycolide) characteristics, (2) the residual poly(vinyl alcohol) (PVA) and (3) the nature of encapsulated proteins, immunoglobulin Y (IgY) or bovine serum albumin, on the microspheres phagocytosis efficiency. The phagocytosis evaluation was performed by flow cytometry and has allowed a screening of microspheres formulations. The hydrophilicity of microspheres resulting from the nature of the polymer and/ or from the residual hydrophilic surface agent (PVA) led to a decrease of phagocytosis intensity. The phagocytosis results of IgY-loaded microspheres strongly suggested that the phagocytosis was increased when the phagocytic cell possessed a receptor for this protein on its surface.
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Particle structures smaller than 5 μm are taken up by APCs such as dendritic cells and macrophages and transported directly to the lymph node, where encapsulated antigen can be presented to T cells.
“Particle structures smaller than 5 μm are taken up by APCs, such as dendritic cells (DCs) and macrophages [59–61], and are transported directly to the lymph node [58], where the encapsulated antigen can be presented to T cells.”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [59]
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- Authors
- Torché, A.-M., Le Corre, P., Albina, E., & Le Verge, R.
- Affiliations
- Université Rennes 1 (FR)
- Corresponding author
- Torché, Anne-Marie
- ORCID
- Albina, Emmanuel
- Venue
- Journal of Drug Targeting
- Publisher
- Informa UK Limited
- Published
- 1999-01
- Volume
- 7
- Issue
- 5
- Pages
- 343-354
- ISSN
- 1026-7158 (ISSN-L)
- 1061-186X
- 1029-2330
- OpenAlex
- W2017382789
- Cited by
- 50
- References
- 38
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2020: 3 · 2019: 3 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 4 · 2012: 4
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of drug targeting (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Inhalation and Respiratory Drug Delivery
- Keywords
- PLGA microspheres, phagocytosis, alveolar macrophages, pigs, polyvinyl alcohol, protein encapsulation, immunoglobulin Y, bovine serum albumin, copolymer composition, macrophage receptors, drug delivery
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Animals, Flow Cytometry, Immunoglobulins, Microspheres, Phagocytosis, Polyglycolic Acid, Polymers, Polyvinyl Alcohol, Serum Albumin, Bovine, Swine, Macrophages, Alveolar, Lactic Acid
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