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Effects of Light, Oxygen, and pH on the Absorbance of 2,2‐Diphenyl‐1‐picrylhydrazyl
Abstract
ABSTRACT: The absorbance of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) at 517 nm in methanol and acetone decreased by 20 and 35% for 120 min at 25 °C under light, respectively; in the dark it did not change significantly for 150 min. Decomposition of DPPH under 21% oxygen after 90 min under light was significantly higher than that under 1% oxygen. Absorbance of DPPH in pH 4 buffer solution in methanol, and in pH 10 buffer solution in acetone, decreased by 55 and 80%, respectively, under light for 120 min. The evaluation of antioxidant activity by the changes of DPPH absorbance should be carefully interpreted since the absorbance of DPPH at 517 nm is decreased by light, oxygen, pH, and type of solvent in addition to the antioxidant.
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Citations
Cited by
Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method
Scherer, R., & Godoy, H. T. (2009) · Food Chemistry · doi:10.1016/j.foodchem.2008.06.026 · Cited by 865
Review of methods to determine antioxidant capacities
Karadag, A., Ozcelik, B., & Saner, S. (2009) · Food Analytical Methods · doi:10.1007/s12161-008-9067-7 · Cited by 753
Ryu, B., Qian, Z.-J., Kim, M.-M., Nam, K. W., & Kim, S.-K. (2009) · Radiation Physics and Chemistry · doi:10.1016/j.radphyschem.2008.09.001 · Cited by 113
Metadata
- Authors
- Ozcelik, B., Lee, J. H., & Min, D. B.
- Venue
- Journal of Food Science
- Publisher
- Wiley
- Published
- 2003-03
- Volume
- 68
- Issue
- 2
- Pages
- 487-490
- ISSN
- 0022-1147 (ISSN-L)
- 1750-3841
- OpenAlex
- W1994083113
- Cited by
- 253
- References
- 0
- FWCI
- 2.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ozcelik, B., Lee, J. H., & Min, D. B. (2003). Effects of Light, Oxygen, and pH on the Absorbance of 2,2‐Diphenyl‐1‐picrylhydrazyl. Journal of Food Science. https://doi.org/10.1111/j.1365-2621.2003.tb05699.x
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