The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying
Abstract
AIMS: The effects of protective agents, rehydration media and freezing temperature on the viabilities of Lactobacillus brevis and Oenococcus oeni H-2 when subjected to freeze-drying were investigated. METHODS AND RESULTS: Several protectants and rehydration media were tested to improve the survival after freeze-drying. The cells were also frozen at -65 and -20 degrees C to check the effect of freezing temperature on the viability. CONCLUSIONS: The best protectant and rehydration medium to obtain the highest viability after freeze-drying varied with the species of bacteria. Yeast extract (4.0%) and sodium glutamate (2.5% ) gave maximum viability of L. brevis and O. oeni (67.8% and 53.6% respectively). The highest survival of L. brevis and O. oeni were obtained when rehydrated with 10% sucrose and MGY medium respectively. When the bacterial cells were frozen quickly (-65 degrees C) than slowly (-20 degrees C), L. brevis and O. oeni both showed increased viability after freeze-drying. SIGNIFICANCE AND IMPACT OF THE STUDY: The viabilities of L. brevis and O. oeni after freeze-drying were shown to be strain specific and dependent on protective agents, rehydration media and freezing temperature.
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Cites
Practical Implications of Malolactic Fermentation: A Review
Davis, C. R., Wibowo, D., Eschenbruch, R., Lee, T. H., & Fleet, G. H. (1985) · American Journal of Enology and Viticulture · doi:10.5344/ajev.1985.36.4.290 · Cited by 423
Cited by
Alternative Drying Processes for the Industrial Preservation of Lactic Acid Starter Cultures
Santivarangkna, C., Kulozik, U., & Foerst, P. (2007) · Biotechnology Progress · doi:10.1021/bp060268f · Cited by 329
Broeckx, G., Vandenheuvel, D., Claes, I. J. J., Lebeer, S., & Kiekens, F. (2016) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2016.04.002 · Cited by 328
Cassani, L., Gomez-Zavaglia, A., & Simal-Gandara, J. (2020) · Food Research International · doi:10.1016/j.foodres.2019.108852 · Cited by 133
Wang, J., Wu, P., Dhital, S., Yu, A., & Chen, X. D. (2025) · Foods · doi:10.3390/foods14101817 · Cited by 49
Metadata
- Authors
- Zhao, G., & Zhang, G.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-08
- Volume
- 99
- Issue
- 2
- Pages
- 333-338
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W2017543991
- Cited by
- 162
- References
- 1
- FWCI
- 10.18
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zhao, G., & Zhang, G. (2005). Effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying. Journal of Applied Microbiology. https://doi.org/10.1111/j.1365-2672.2005.02587.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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