Sample preparation procedures for biological atomic force microscopy
Abstract
Summary: Since the late 1980s, atomic force microscopy (AFM) has been increasingly used in biological sciences and it is now established as a versatile tool to address the structure, properties and functions of biological specimens. AFM is unique in that it provides three‐dimensional images of biological structures, including biomolecules, lipid films, 2D protein crystals and cells, under physiological conditions and with unprecedented resolution. A crucial prerequisite for successful, reliable biological AFM is that the samples need to be well attached to a solid substrate using appropriate, nondestructive methods. In this review, we discuss common techniques for immobilizing biological specimens for AFM studies.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
To ensure good results of AFM measurement, the specimen should be strongly attached to the solid support surface to resist lateral forces exerted by the scanning tip which could move, sweep or drag the specimen producing important artifacts concerning nano-systems morphology.
“In order to ensure good results of the measurement, the specimen should be strongly attached to the solid support surface to resist the lateral forces exerted by the scanning tip which could move, sweep or drag the specimen producing important artifacts concerning nano-systems morphology [151].”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [151]
Cited by
Sorted by citations
Ghezzi, M., Pescina, S., Padula, C., Santi, P., Del Favero, E., Cantù, L., & Nicoli, S. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.02.031 · Cited by 1,135 · see citing works
Applications of biosensing atomic force microscopy in monitoring drug and nanoparticle delivery
Lamprecht, C., Hinterdorfer, P., & Ebner, A. (2014) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2014.917078 · Cited by 46 · see citing works
- Authors
- EL KIRAT, K., BURTON, I., DUPRES, V., & DUFRENE, Y. F.
- Affiliations
- UCLouvain (BE)
- Venue
- Journal of Microscopy
- Publisher
- Wiley
- Published
- 2005-06
- Volume
- 218
- Issue
- 3
- Pages
- 199-207
- ISSN
- 0022-2720 (ISSN-L)
- 1365-2818
- OpenAlex
- W1981294609
- Cited by
- 130
- References
- 0
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 6 · 2021: 7 · 2020: 5 · 2019: 5 · 2018: 5 · 2017: 12 · 2016: 8 · 2015: 5 · 2014: 5 · 2013: 9 · 2012: 3
- FWCI
- 0.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Microscopy (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- DIAL (Catholic University of Leuven) (submittedVersion)
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Keywords
- sample preparation, biological specimen, 3D imaging, lipid films, protein crystals, cells
- MeSH terms
- Animals, Cells, DNA, Lipids, Proteins, Specimen Handling, Microscopy, Atomic Force
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
