The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Conservation in a cup of water: estimating biodiversity and population abundance from environmental DNA
Open accessAbstract
Three mantras often guide species and ecosystem management: (i) for preventing invasions by harmful species, ‘early detection and rapid response’; (ii) for conserving imperilled native species, ‘protection of biodiversity hotspots’; and (iii) for assessing biosecurity risk, ‘an ounce of prevention equals a pound of cure.’ However, these and other management goals are elusive when traditional sampling tools (e.g. netting, traps, electrofishing, visual surveys) have poor detection limits, are too slow or are not feasible. One visionary solution is to use an organism’s DNA in the environment (eDNA), rather than the organism itself, as the target of detection. In this issue of Molecular Ecology, Thomsen et al. (2012) provide new evidence demonstrating the feasibility of this approach, showing that eDNA is an accurate indicator of the presence of an impressively diverse set of six aquatic or amphibious taxa including invertebrates, amphibians, a fish and a mammal in a wide range of freshwater habitats. They are also the first to demonstrate that the abundance of eDNA, as measured by qPCR, correlates positively with population abundance estimated with traditional tools. Finally, Thomsen et al. (2012) demonstrate that next‐generation sequencing of eDNA can quantify species richness. Overall, Thomsen et al. (2012) provide a revolutionary roadmap for using eDNA for detection of species, estimates of relative abundance and quantification of biodiversity.
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Cites
Funk, D. J., & Omland, K. E. (2003) · Annual Review of Ecology, Evolution, and Systematics · doi:10.1146/annurev.ecolsys.34.011802.132421 · Cited by 2,237
Species detection using environmental DNA from water samples
Ficetola, G. F., Miaud, C., Pompanon, F., & Taberlet, P. (2008) · Biology Letters · doi:10.1098/rsbl.2008.0118 · Cited by 1,707
“Sight‐unseen” detection of rare aquatic species using environmental DNA
Jerde, C. L., Mahon, A. R., Chadderton, W. L., & Lodge, D. M. (2011) · Conservation Letters · doi:10.1111/j.1755-263x.2010.00158.x · Cited by 1,304
Self-citationMonitoring endangered freshwater biodiversity using environmental DNA
THOMSEN, P. F., KIELGAST, J., IVERSEN, L. L., WIUF, C., RASMUSSEN, M., GILBERT, M. T. P., ORLANDO, L., & WILLERSLEV, E. (2012) · Molecular Ecology · doi:10.1111/j.1365-294x.2011.05418.x · Cited by 1,184
The extraction and purification of microbial DNA from sediments
Ogram, A., Sayler, G. S., & Barkay, T. (1987) · Journal of Microbiological Methods · doi:10.1016/0167-7012(87)90025-x · Cited by 783
Persistence of Environmental DNA in Freshwater Ecosystems
Dejean, T., Valentini, A., Duparc, A., Pellier-Cuit, S., Pompanon, F., Taberlet, P., & Miaud, C. (2011) · PLoS ONE · doi:10.1371/journal.pone.0023398 · Cited by 685
Diverse Plant and Animal Genetic Records from Holocene and Pleistocene Sediments
Willerslev, E., Hansen, A. J., Binladen, J., Brand, T. B., Gilbert, M. T. P., Shapiro, B., Bunce, M., Wiuf, C., Gilichinsky, D. A., & Cooper, A. (2003) · Science · doi:10.1126/science.1084114 · Cited by 599
Goldberg, C. S., Pilliod, D. S., Arkle, R. S., & Waits, L. P. (2011) · PLoS ONE · doi:10.1371/journal.pone.0022746 · Cited by 592
Taylor, C. F., Field, D., Sansone, S.-A., Aerts, J., Apweiler, R., Ashburner, M., Ball, C. A., Binz, P.-A., Bogue, M., Booth, T., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt.1411 · Cited by 558 · cited 1×
“For laboratory protocols, adherence to the minimum information reporting guidelines for qPCR and metagenetics (Taylor et al. 2008) would at least make it more possible to compare protocols among publications even if the specific effects of different protocols were unknown.”
MethodDarling, J. A., & Mahon, A. R. (2011) · Environmental Research · doi:10.1016/j.envres.2011.02.001 · Cited by 501
Self-citation
Cited by
Environmental Conditions Influence eDNA Persistence in Aquatic Systems
Barnes, M. A., Turner, C. R., Jerde, C. L., Renshaw, M. A., Chadderton, W. L., & Lodge, D. M. (2014) · Environmental Science & Technology · doi:10.1021/es404734p · Cited by 939
Self-citationFish environmental DNA is more concentrated in aquatic sediments than surface water
Turner, C. R., Uy, K. L., & Everhart, R. C. (2015) · Biological Conservation · doi:10.1016/j.biocon.2014.11.017 · Cited by 555
Self-citationFactors influencing detection of eDNA from a stream‐dwelling amphibian
Pilliod, D. S., Goldberg, C. S., Arkle, R. S., & Waits, L. P. (2014) · Molecular Ecology Resources · doi:10.1111/1755-0998.12159 · Cited by 517
Biggs, J., Ewald, N., Valentini, A., Gaboriaud, C., Dejean, T., Griffiths, R. A., Foster, J., Wilkinson, J. W., Arnell, A., Brotherton, P., et al. (2015) · Biological Conservation · doi:10.1016/j.biocon.2014.11.029 · Cited by 503
Using Environmental DNA to Estimate the Distribution of an Invasive Fish Species in Ponds
Takahara, T., Minamoto, T., & Doi, H. (2013) · PLoS ONE · doi:10.1371/journal.pone.0056584 · Cited by 484
Metadata
- Authors
- LODGE, D. M., TURNER, C. R., JERDE, C. L., BARNES, M. A., CHADDERTON, L., EGAN, S. P., FEDER, J. L., MAHON, A. R., & PFRENDER, M. E.
- Venue
- Molecular Ecology
- Publisher
- Wiley
- Published
- 2012-05-24
- Volume
- 21
- Issue
- 11
- Pages
- 2555-2558
- ISSN
- 0962-1083 (ISSN-L)
- 1365-294X
- OpenAlex
- W2152383265
- Cited by
- 313
- References
- 18
- FWCI
- 16.97
- Type
- journal article
- Language
- en
- Open access
- bronze
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- LODGE, D. M., TURNER, C. R., JERDE, C. L., BARNES, M. A., CHADDERTON, L., EGAN, S. P., FEDER, J. L., MAHON, A. R., & PFRENDER, M. E. (2012). Conservation in a cup of water: estimating biodiversity and population abundance from environmental DNA. Molecular Ecology. https://doi.org/10.1111/j.1365-294x.2012.05600.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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