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Cited by 183

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family

McAdam, R. A., Hermans, P. W. M., van Soolingen, D., Zainuddin, Z. F., Catty, D., van Embden, J. D. A., Dale, J. W.·1990·Molecular Microbiology

Abstract

Summary: A repetitive element (IS986), previously isolated from Mycobacterium tuberculosis and shown to detect multiple restriction fragment‐length polymorphisms (RFLPs), has been sequenced. It consists of a potential insertion sequence of 1358bp, with 30‐bp inverted repeat ends. IS986 has four potentially significant open reading frames (ORFs): ORFa1, ORFa2 and ORFb on one strand and ORFc on the complementary strand. The sequences of the potential translated products identify IS986 as a member of the IS3 family, with an apparent frameshift between ORFa1 and ORFa2. IS986 has potential as a highly specific probe for detection and typing of M. tuberculosis, as well as for transposon mutagenesis of mycobacteria. The sequence of IS986 is virtually identical to that of another recently described element, IS6110 (Thierry et al., 1990).

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Crossref · 10.1111/j.1365-2958.1990.tb02073.x

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Metadata

Authors
McAdam, R. A., Hermans, P. W. M., van Soolingen, D., Zainuddin, Z. F., Catty, D., van Embden, J. D. A., & Dale, J. W.
Venue
Molecular Microbiology
Publisher
Wiley
Published
1990-09
Volume
4
Issue
9
Pages
1607-1613
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
OpenAlex
W1987355762
Cited by
183
References
1
FWCI
14.70
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family. https://app.cochat.ai/papers/10.1111/j.1365-2958.1990.tb02073.x
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McAdam, R. A., Hermans, P. W. M., van Soolingen, D., Zainuddin, Z. F., Catty, D., van Embden, J. D. A., & Dale, J. W. (1990). Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.1990.tb02073.x

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