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Cited by 356

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Nature of DNA polymorphism in the direct repeat cluster of Mycobacterium tuberculosis; application for strain differentiation by a novel typing method

Groenen, Peter M. A., Bunschoten, Annelies E., Soolingen, Dick van, Errtbden, Jan D. A. van·1993·Molecular Microbiology
DOI 10.1111/j.1365-2958.1993.tb00976.x
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Abstract

Summary: Mycobacterium tuberculosis complex strains contain a unique chromosomal region, which consists of multiple 36bp direct repeats (DRs), which are interspersed by unique spacers 35 to 41 bp in length. In this study we investigated the nature of the DNA polymorphism of this DR cluster by sequencing part of this region in a large number of M. tuberculosis complex strains. Two types of genetic rearrangements were observed. One type consists of the variation in one or a few discrete, contiguous DRs plus spacer sequences. This variation is probably driven by homologous recombination between adjacent or distant DRs. The other type of polymorphism is probably driven by transpositional events of the insertion sequence, IS6110, which is almost invariably present in the DR cluster of M. tuberculosis complex strains. Based on the nature of the DNA polymorphism in the DR cluster, we developed a novel method of strain differentiation, direct variable repeat polymer chain reaction (DVR‐PCR), which enables typing of individual M. tuberculosis strains in a single PCR. The method allows an excellent differentiation of epidemiologically unrelated isolates and, in principle, the DVR‐PCR allows the detection of M. tuberculosis and strain differentiation at the same time.

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  • CRISPR loci have a high degree of polymorphism in different strains of the same species of pathogenic bacteria, enabling identification of bacterial strains in clinical conditions.

    “As it turned out, CRISPR loci had a high degree of polymorphism in different strains of the same species of pathogenic bacteria, which enabled the identification of bacterial strains in clinical conditions.”

    From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [2]

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Research works printed but not linked: 24

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Metadata

Authors
Groenen, P. M. A., Bunschoten, A. E., Soolingen, D. V., & Errtbden, J. D. A. V.
Venue
Molecular Microbiology
Publisher
Wiley
Published
1993-12
Volume
10
Issue
5
Pages
1057-1065
ISSN
  • 0950-382X
  • 1365-2958
DOI
10.1111/j.1365-2958.1993.tb00976.x
Cited by
356
References
1
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 4713 d)

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