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23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody Titre
Abstract
Summary. High titred monospecific antibodies are obtained in rabbits injecting as little as 25 μg pure antigen per kg body weight. For polyspecific antibodies the dose is increased 10 times. The immunization schedule is described in detail. A procedure is given for the isolation of the immunoglobulins from antiserum by salting out and ion exchange chromatography. Finally antibody titres as measured by 4 different titration methods are compared using anti‐human IgG from rabbits as a model.
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Citations
Cited by
Oertel, W. H., Schmechel, D. E., Tappaz, M. L., & Kopin, I. J. (1981) · Neuroscience · doi:10.1016/0306-4522(81)90113-5 · Cited by 419
Schousboe, I. (1985) · Blood · doi:10.1182/blood.v66.5.1086.1086 · Cited by 366
On the mechanism of membrane damage by Staphylococcus aureus alpha-toxin.
Füssle, R., Bhakdi, S., Sziegoleit, A., Tranum-Jensen, J., Kranz, T., & Wellensiek, H. J. (1981) · The Journal of cell biology · doi:10.1083/jcb.91.1.83 · Cited by 366
Development and storage-protein synthesis in Brassica napus L. embryos in vivo and in vitro
Crouch, M. L., & Sussex, I. M. (1981) · Planta · doi:10.1007/bf00385319 · Cited by 273
Stress and mortality in a small marsupial (Antechinus stuartii, Macleay)
Bradley, A. J., McDonald, I. R., & Lee, A. K. (1980) · General and Comparative Endocrinology · doi:10.1016/0016-6480(80)90122-7 · Cited by 261
Metadata
- Authors
- HARBOE, N., & INGILD, A.
- Venue
- Scandinavian Journal of Immunology
- Publisher
- Wiley
- Published
- 1973-10
- Volume
- 2
- Issue
- s1
- Pages
- 161-164
- ISSN
- 0300-9475 (ISSN-L)
- 1365-3083
- OpenAlex
- W2080656627
- Cited by
- 854
- References
- 0
- FWCI
- 25.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: 23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody Titre. https://app.cochat.ai/papers/10.1111/j.1365-3083.1973.tb03798.x
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- HARBOE, N., & INGILD, A. (1973). 23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody Titre. Scandinavian Journal of Immunology. https://doi.org/10.1111/j.1365-3083.1973.tb03798.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
