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Cited by 259

The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.

Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection

Sato, Shigeru, Soga, Tomoyoshi, Nishioka, Takaaki, Tomita, Masaru·2004·The Plant Journal

Abstract

Summary: The study of the metabolomics of primary metabolites using conventional chemical analyses requires a high‐throughput method. Chemical derivatizations are a prerequisite for gas‐chromatographic separation, and a large sample quantity is needed for liquid‐chromatographic separation and nuclear magnetic resonance detection systems. Recently, we have developed a capillary electrophoresis–mass spectrometry (CE–MS) technology that can simultaneously quantify a large number of primary metabolites, using only a small quantity of samples, and without any chemical derivatizations. Parallel use of a capillary electrophoresis–diode array detector (CE–DAD) system further enables almost all water‐soluble intracellular metabolites to be analyzed. We demonstrate, with rice leaves, a simple and rapid method of sample preparation for CE analysis; using this method, we have successfully measured the levels of 88 main metabolites involved in glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway, photorespiration, and amino acid biosynthesis.

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Crossref · 10.1111/j.1365-313x.2004.02187.x

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Metadata

Authors
Sato, S., Soga, T., Nishioka, T., & Tomita, M.
Venue
The Plant Journal
Publisher
Wiley
Published
2004-08-04
Volume
40
Issue
1
Pages
151-163
ISSN
  • 0960-7412 (ISSN-L)
  • 1365-313X
OpenAlex
W1484632047
Cited by
259
References
27
FWCI
19.43
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection. https://app.cochat.ai/papers/10.1111/j.1365-313x.2004.02187.x
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Sato, S., Soga, T., Nishioka, T., & Tomita, M. (2004). Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2004.02187.x

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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