Identification of the Arabidopsis dry2/sqe1‐5 mutant reveals a central role for sterols in drought tolerance and regulation of reactive oxygen species
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Summary: Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3‐oxidosqualene, the precursor of cyclic triterpenoids. Here we report that the Arabidopsis drought hypersensitive/squalene epoxidase 1‐5 (dry2/sqe1‐5) mutant, identified by its extreme hypersensitivity to drought stress, has altered stomatal responses and root defects because of a point mutation in the SQUALENE EPOXIDASE 1 (SQE1) gene. GC‐MS analysis indicated that the dry2/sqe1‐5 mutant has altered sterol composition in roots but wild‐type sterol composition in shoots, indicating an essential role for SQE1 in root sterol biosynthesis. Importantly, the stomatal and root defects of the dry2/sqe1‐5 mutant are associated with altered production of reactive oxygen species. As RHD2 NADPH oxidase is de‐localized in dry2/sqe1‐5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, stomatal responses and drought tolerance.
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Only one other null eukaryotic mutation of Sqle has been identified, resulting in an inability for plants to tolerate drought stress.
“Only one other null eukaryotic mutation of Sqle has been identified, resulting in an inability for plants to tolerate drought stress26,27.”
From A suppressor screen in Mecp2 mutant mice implicates cholesterol metabolism in Rett syndrome (2013) · cites this paper as [27]
The section on drought hypersensitivity reports that the Arabidopsis dry2 mutant is extremely sensitive to dehydration, and the section identifying DRY2 as a squalene epoxidase links that phenotype to SQE1. But the paper calls dry2/sqe1-5 a hypomorphic allele, not a null mutation. It also discusses a separate loss-of-function allele, sqe1-3, without reporting its drought tolerance or establishing that it is the only other null eukaryotic SQE mutation. Page numbers were not available in our copy. (LLM, conf=0.88)
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Schaller, H., Grausem, B., Benveniste, P., Chye, M. L., Tan, C. T., Song, Y. H., & Chua, N. H. (1995) · Plant Physiology · doi:10.1104/pp.109.3.761 · Cited by 230 · see citing works
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- Babiychuk, E., Bouvier-Nave ´, P., Compagnon, V., Suzuki, M., Muranaka, T., Van Montagu, M., Kushnir, S. and Schaller, H. (2008) Allelic mutant series reveal distinct functions for Arabidopsis cycloartenol synthase 1 in cell viability and plastid biogenesis. Proc. Natl Acad. Sci. USA, 105, 3163-3168.
- Orozco-Cardenas, M. and Ryan, C.A. (1999) Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadeca- noid pathway. Proc. Natl Acad. Sci. USA, 96, 6553-6557.
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A suppressor screen in Mecp2 mutant mice implicates cholesterol metabolism in Rett syndrome
Buchovecky, C. M., Turley, S. D., Brown, H. M., Kyle, S. M., McDonald, J. G., Liu, B., Pieper, A. A., Huang, W., Katz, D. M., Russell, D. W., et al. (2013) · Nature Genetics · doi:10.1038/ng.2714 · Cited by 234 · see citing works
Sabater-Jara, A. B., & Pedreño, M. A. (2013) · Plant Cell, Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-013-0320-4 · Cited by 35 · see citing works
Singh, R., Pandey, N., Kumar, A., & Shirke, P. A. (2016) · Protoplasma · doi:10.1007/s00709-015-0800-y · Cited by 31 · see citing works
- Authors
- Posé, D., Castanedo, I., Borsani, O., Nieto, B., Rosado, A., Taconnat, L., Ferrer, A., Dolan, L., Valpuesta, V., & Botella, M. A.
- Affiliations
- Universidad de Málaga (ES)
- Universidad de la República de Uruguay (UY)
- Universitat de Barcelona (ES)
- University of California, Riverside (US)
- John Innes Centre (GB)
- Corresponding author
- Botella, Miguel A.
- ORCID
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2009-06-25
- Volume
- 59
- Issue
- 1
- Pages
- 63-76
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2098546767
- Cited by
- 145
- References
- 1
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 14 · 2023: 12 · 2022: 7 · 2021: 12 · 2020: 12 · 2019: 7 · 2018: 5 · 2017: 10 · 2016: 11 · 2015: 8 · 2014: 8 · 2013: 9 · 2012: 6
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Environment Research Council (NE/C510732/1)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Surface Properties and Treatments
- Keywords
- squalene epoxidase, sterols, drought tolerance, reactive oxygen species, NADPH oxidase, stomatal response, root hairs, Arabidopsis thaliana
- MeSH terms
- Dehydration, Genetic Complementation Test, Mutation, Sterols, Stress, Physiological, Arabidopsis, Reactive Oxygen Species, Gene Expression Regulation, Plant, Plant Roots, DNA, Plant, NADPH Oxidases, Oligonucleotide Array Sequence Analysis, Arabidopsis Proteins, Squalene Monooxygenase, Plant Stomata, Droughts
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