Cited by 230Plant biochemistry and biosynthesisLipid metabolism and biosynthesisCholesterol and Lipid Metabolism

Expression of the Hevea brasiliensis (H.B.K.) Mull. Arg. 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase 1 in Tobacco Results in Sterol Overproduction

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Schaller, H., Grausem, B., Benveniste, P., Chye, M. L., Tan, C. T., Song, Y. H., Chua, N. H.·1995·Plant Physiology

Abstract

A genomic fragment encoding one (HMGR1) of the three 3-hydroxy-3-methylglutaryl coenzyme A reductases (HMGRs) from Hevea brasiliensis (H.B.K.) Mull. Arg. (M.-L. Chye, C.-T. Tan, N.-H. Chua [1992] Plant Mol Biol 19: 473-484) was introduced into Nicotiana tabacum L. cv xanthi via Agrobacterium transformation to study the influence of the hmg1 gene product on plant isoprenoid biosynthesis. Transgenic plants were morphologically indistinguishable from control wild-type plants and displayed the same developmental pattern. Transgenic lines showed an increase in the level of total sterols up to 6-fold, probably because of an increased expression level of hmg1 mRNA and a corresponding increased enzymatic activity for HMGR, when compared with the level of total sterols from control lines not expressing the hmg1 transgene. In addition to the pathway end products, campesterol, sitosterol, and stigmasterol, some biosynthetic intermediates such as cycloartenol also accumulated in transgenic tissues. Most of the overproduced sterols were detected as steryl-esters and were likely to be stored in cytoplasmic lipid bodies. These data strongly support the conclusion that plant HMGR is a key limiting enzyme in phytosterol biosynthesis.

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Crossref · 10.1104/pp.109.3.761

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Authors
Schaller, H., Grausem, B., Benveniste, P., Chye, M. L., Tan, C. T., Song, Y. H., & Chua, N. H.
Affiliations
Institut de Biologie Moléculaire des Plantes (FR)
Venue
Plant Physiology
Published
1995-11-01
Volume
109
Issue
3
Pages
761-770
ISSN
  • 0032-0889 (ISSN-L)
  • 1532-2548
OpenAlex
W1607854320
Cited by
230
References
33
Citations by year
2026: 4 · 2025: 3 · 2024: 7 · 2023: 4 · 2022: 5 · 2021: 1 · 2020: 10 · 2019: 10 · 2018: 7 · 2017: 13 · 2016: 5 · 2015: 10 · 2014: 11 · 2013: 13 · 2012: 9
FWCI
1.86
Type
journal article
Language
en
Open access
Locations
  • PLANT PHYSIOLOGY (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • National University of Singapore (submittedVersion)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
Keywords
3-hydroxy-3-methylglutaryl-CoA reductase, Hevea brasiliensis, sterol biosynthesis, phytosterols, isoprenoid biosynthesis, transgenic tobacco, Agrobacterium-mediated transformation, cycloartenol, sterol esters, lipid droplets, campesterol, sitosterol, stigmasterol

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