Expression of the Hevea brasiliensis (H.B.K.) Mull. Arg. 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase 1 in Tobacco Results in Sterol Overproduction
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A genomic fragment encoding one (HMGR1) of the three 3-hydroxy-3-methylglutaryl coenzyme A reductases (HMGRs) from Hevea brasiliensis (H.B.K.) Mull. Arg. (M.-L. Chye, C.-T. Tan, N.-H. Chua [1992] Plant Mol Biol 19: 473-484) was introduced into Nicotiana tabacum L. cv xanthi via Agrobacterium transformation to study the influence of the hmg1 gene product on plant isoprenoid biosynthesis. Transgenic plants were morphologically indistinguishable from control wild-type plants and displayed the same developmental pattern. Transgenic lines showed an increase in the level of total sterols up to 6-fold, probably because of an increased expression level of hmg1 mRNA and a corresponding increased enzymatic activity for HMGR, when compared with the level of total sterols from control lines not expressing the hmg1 transgene. In addition to the pathway end products, campesterol, sitosterol, and stigmasterol, some biosynthetic intermediates such as cycloartenol also accumulated in transgenic tissues. Most of the overproduced sterols were detected as steryl-esters and were likely to be stored in cytoplasmic lipid bodies. These data strongly support the conclusion that plant HMGR is a key limiting enzyme in phytosterol biosynthesis.
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HMGR is a rate-limiting enzyme of the mevalonate pathway and HMGR expression limits artemisinin formation in A. annua.
“HMGR is a rate-limiting enzyme of the mevalonate pathway [24,25], and recently it has been shown that HMGR expression limits artemisinin formation in A. annua [26].”
From Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL (2011) · cites this paper as [25]
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- Authors
- Schaller, H., Grausem, B., Benveniste, P., Chye, M. L., Tan, C. T., Song, Y. H., & Chua, N. H.
- Affiliations
- Institut de Biologie Moléculaire des Plantes (FR)
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-11-01
- Volume
- 109
- Issue
- 3
- Pages
- 761-770
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- PMC
- PMC161375
- OpenAlex
- W1607854320
- Cited by
- 230
- References
- 33
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 7 · 2023: 4 · 2022: 5 · 2021: 1 · 2020: 10 · 2019: 10 · 2018: 7 · 2017: 13 · 2016: 5 · 2015: 10 · 2014: 11 · 2013: 13 · 2012: 9
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- National University of Singapore (submittedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- 3-hydroxy-3-methylglutaryl-CoA reductase, Hevea brasiliensis, sterol biosynthesis, phytosterols, isoprenoid biosynthesis, transgenic tobacco, Agrobacterium-mediated transformation, cycloartenol, sterol esters, lipid droplets, campesterol, sitosterol, stigmasterol
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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