Rubisco activase is a key regulator of non‐steady‐state photosynthesis at any leaf temperature and, to a lesser extent, of steady‐state photosynthesis at high temperature

Abstract

Summary: The role of Rubisco activase in steady‐state and non‐steady‐state photosynthesis was analyzed in wild‐type (Oryza sativa) and transgenic rice that expressed different amounts of Rubisco activase. Below 25°C, the Rubisco activation state and steady‐state photosynthesis were only affected when Rubisco activase was reduced by more than 70%. However, at 40°C, smaller reductions in Rubisco activase content were linked to a reduced Rubisco activation state and steady‐state photosynthesis. As a result, overexpression of maize Rubisco activase in rice did not lead to an increase of the Rubisco activation state, nor to an increase in photosynthetic rate below 25°C, but had a small stimulatory effect at 40°C. On the other hand, the rate at which photosynthesis approached the steady state following an increase in light intensity was rapid in Rubisco activase‐overexpressing plants, intermediate in the wild‐type, and slowest in antisense plants at any leaf temperature. In Rubisco activase‐overexpressing plants, Rubisco activation state at low light was maintained at higher levels than in the wild‐type. Thus, rapid regulation by Rubisco activase following an increase in light intensity and/or maintenance of a high Rubisco activation state at low light would result in a rapid increase in Rubisco activation state and photosynthetic rate following an increase in light intensity. It is concluded that Rubisco activase plays an important role in the regulation of non‐steady‐state photosynthesis at any leaf temperature and, to a lesser extent, of steady‐state photosynthesis at high temperature.