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Two-Dimensional Gel Analysis of Histones in Acid Extracts of Nuclei, Cells, and Tissues
Abstract
Two-dimensional gel analysis of histones from extracts of nuclei, cells, and tissues is described. A discontinuous buffer system concentrates the sample was used to increase resolution in both dimensions and also to allow the direct loading of HCl extracts of chromatin, nuclei, cells, and tissues. Stained one-dimensional gels are used as sample gels for the second dimension, cetylltrimethylammonium bromide being used to solubilize the proteins in the dye-protein complex. These methods enable one to purify proteins through acetic acid/urea/Triton, acetic acid/urea, and sodium dodecyl sulfate gels without eluting them from the gels. The method is also compatible with the use of protamine to displace histones from nuclei and nucleosomes separated in chromatin gels.
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Cited by
DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139
Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S., & Bonner, W. M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.10.5858 · Cited by 5,438
Self-citationThermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85
Finley, D., Ciechanover, A., & Varshavsky, A. (1984) · Cell · doi:10.1016/0092-8674(84)90299-x · Cited by 522
Smith, E. R., Pannuti, A., Gu, W., Steurnagel, A., Cook, R. G., Allis, C. D., & Lucchesi, J. C. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.1.312-318.2000 · Cited by 316
Histone acetylation and globin gene switching
Hebbes, T. R., Thorne, A. W., Clayton, A. L., & Crane-Robinson, C. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.5.1017 · Cited by 113
Marchant, P., Dredar, S., Manneh, V., Alshabanah, O., Matthews, H., Fries, D., & Blankenship, J. (1989) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(89)90170-7 · Cited by 23
Metadata
- Authors
- BONNER, W. M., WEST, M. H. P., & STEDMAN, J. D.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1980-08
- Volume
- 109
- Issue
- 1
- Pages
- 17-23
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1984769155
- Cited by
- 241
- References
- 0
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Two-Dimensional Gel Analysis of Histones in Acid Extracts of Nuclei, Cells, and Tissues. https://app.cochat.ai/papers/10.1111/j.1432-1033.1980.tb04762.x
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- BONNER, W. M., WEST, M. H. P., & STEDMAN, J. D. (1980). Two-Dimensional Gel Analysis of Histones in Acid Extracts of Nuclei, Cells, and Tissues. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1980.tb04762.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
