The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Purification of Mouse Immunoglobulin Heavy‐Chain Messenger RNAs from Total Myeloma Tumor RNA
Abstract
A procedure is described for the large‐scale purification of light (L) and heavy (H) chain mRNAs from plasmacytomas produced in mice. Intact RNA is selectively precipitated in high yield from frozen tumors homogenized in 3 M LiCl and 6 M urea. L and H‐chain mRNAs were purified by oligo(dT)‐cellulose chromatography and either sucrose gradient centrifugation in conditions preventing aggregation or by means of high‐resolution preparative gel electrophoresis under non‐denaturing conditions. γ2a and α H‐chain mRNAs sedimented as major components at 15.5 S and 16.5 S respectively, when L‐chain mRNAs sedimented as 12‐S species. H‐chain mRNAs isolated by continuous elution during preparative gel electrophoresis were completely separated from both L‐chain mRNA and residual 18‐S rRNA, and migrated as single components of 1900 ± 50 nucleotides on analytical denaturing gels. The partially purified H‐chain mRNAs were translated into major components of molecular weights of 56000 (γ2a) and 60000 (α) in an mRNA‐dependent rabbit reticulocyte lysate, whereas L‐chain mRNAs yielded polypeptides of molecular weights of 25000 (λ) and 27000 (к). Up to 95% of the translation products directed by the purified mRNAs were immunoprecipitated using specific antisera. The purity of L and H‐chain mRNAs was assessed by hybridization of corresponding cDNAs with excess recombinant plasmid DNA. The results indicated a minimum purity of 47% (γ2a), 62% (α), for H‐chain mRNAs and 60% (к), for L‐chain mRNAs.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
High resolution two-dimensional electrophoresis of proteins.
O'Farrell, P. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41496-8 · Cited by 19,246
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
BONNER, W. M., & LASKEY, R. A. (1974) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1974.tb03599.x · Cited by 8,589
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.
Bolívar, F. G., Rl, R., Pj, G., Betlach, M. C., Hl, H., Boyer, H. W., Crosa, J. H., & Falkow, S. (1992) · PubMed · Cited by 4,672
An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates
PELHAM, H. R. B., & JACKSON, R. J. (1976) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1976.tb10656.x · Cited by 3,926
Construction and characterization of new cloning vehicle. II. A multipurpose cloning system
Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., & Falkow, S. (1977) · Gene · doi:10.1016/0378-1119(77)90000-2 · Cited by 3,858
Maniatis, T., Jeffrey, A., & Van deSande, H. (1975) · Biochemistry · doi:10.1021/bi00688a010 · Cited by 1,121
Katz, L., Kingsbury, D. T., & Helinski, D. R. (1973) · Journal of Bacteriology · doi:10.1128/jb.114.2.577-591.1973 · Cited by 496
Traub, P., & Nomura, M. (1968) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.59.3.777 · Cited by 409
Schibler, U., Marcu, K. B., & Perry, R. P. (1978) · Cell · doi:10.1016/0092-8674(78)90072-7 · Cited by 308
Cited by
Brook, J. D., McCurrach, M. E., Harley, H. G., Buckler, A. J., Church, D., Aburatani, H., Hunter, K., Stanton, V. P., Thirion, J.-P., Hudson, T., et al. (1992) · Cell · doi:10.1016/0092-8674(92)90154-5 · Cited by 2,777
Call, K. M., Glaser, T., Ito, C. Y., Buckler, A. J., Pelletier, J., Haber, D. A., Rose, E. A., Kral, A., Yeger, H., Lewis, W. H., et al. (1990) · Cell · doi:10.1016/0092-8674(90)90601-a · Cited by 1,876
MyoD or Myf-5 is required for the formation of skeletal muscle
Rudnicki, M. A., Schnegelsberg, P. N. J., Stead, R. H., Braun, T., Arnold, H.-H., & Jaenisch, R. (1993) · Cell · doi:10.1016/0092-8674(93)90621-v · Cited by 1,731
CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locus
Hark, A. T., Schoenherr, C. J., Katz, D. J., Ingram, R. S., Levorse, J. M., & Tilghman, S. M. (2000) · Nature · doi:10.1038/35013106 · Cited by 1,547
A cellular gene encodes scrapie PrP 27-30 protein
Oesch, B., Westaway, D., Wälchli, M., McKinley, M. P., Kent, S. B. H., Aebersold, R., Barry, R. A., Tempst, P., Teplow, D. B., Hood, L. E., et al. (1985) · Cell · doi:10.1016/0092-8674(85)90333-2 · Cited by 1,513
Metadata
- Authors
- AUFFRAY, C., & ROUGEON, F.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1980-06
- Volume
- 107
- Issue
- 2
- Pages
- 303-314
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1990205754
- Cited by
- 2,627
- References
- 18
- FWCI
- 43.07
- Type
- journal article
- Language
- en
- Open access
- closed
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- AUFFRAY, C., & ROUGEON, F. (1980). Purification of Mouse Immunoglobulin Heavy‐Chain Messenger RNAs from Total Myeloma Tumor RNA. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1980.tb06030.x
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