The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis
Abstract
We describe the use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides). Double-stranded DNA molecules of known length produced either by organic synthesis or by restriction endonuclease digestion of viral DNAs were used as standards. The relative electrophoretic mobilities of these standards were examined on both nondenaturing (aqueous) polyacrylamide gels and on denaturing gels containing 7 M urea or 98% formamide. Electrophoretic mobility of DNA is a linear function of the log of molecular weight if appropriate conditions are used, although exceptions are noted. Chain lengths can be conveniently estimated by using as standards bacteriophage gamma DNA restriction fragments or commercially available tracking dyes.
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Citations
Cites
Nucleotide sequence of the rightward operator of phage lambda.
Maniatis, T., Jeffrey, A., & Kleid, D. G. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.1184 · Cited by 2,030
Self-citationSharp, P. A., Sugden, B., & Sambrook, J. (1973) · Biochemistry · doi:10.1021/bi00740a018 · Cited by 1,551
Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.
Richardson, C. C. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.54.1.158 · Cited by 607
Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
Pribnow, D. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.784 · Cited by 606
The Nucleotide Sequence of the lac Operator
Gilbert, W., & Maxam, A. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.12.3581 · Cited by 485
CLARK, R. J., & FELSENFELD, G. (1971) · Nature New Biology · doi:10.1038/newbio229101a0 · Cited by 459
Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae
Danna, K., & Nathans, D. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.12.2913 · Cited by 397
Full length and discrete partial reverse transcripts of globin and chorion mRNAs
Efstratiadis, A., Maniatis, T., Kafatos, F. C., Jeffrey, A., & Vournakis, J. N. (1975) · Cell · doi:10.1016/0092-8674(75)90157-9 · Cited by 218
Self-citationCIII. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast
Khorana, H. G., Agarwal, K. L., Büchi, H., Caruthers, M. H., Gupta, N. K., Klbppe, K., Kumar, A., Ohtsuka, E., RajBhandary, U. L., van de Sande, J. H., et al. (1972) · Journal of Molecular Biology · doi:10.1016/0022-2836(72)90146-5 · Cited by 200
Weber, H., & Khorana, H. G. (1972) · Journal of Molecular Biology · doi:10.1016/0022-2836(72)90147-7 · Cited by 174
Cited by
Enzymatic in vitro synthesis of globin genes
Efstratiadis, A., Kafatos, F. C., Maxam, A. M., & Maniatis, T. (1976) · Cell · doi:10.1016/0092-8674(76)90027-1 · Cited by 342
Self-citationKreysing, M., Keil, L., Lanzmich, S., & Braun, D. (2015) · Nature Chemistry · doi:10.1038/nchem.2155 · Cited by 169
Changes in Chromatin Structure Associated with Alzheimer’s Disease
Lewis, P. N., Lukiw, W. J., De Boni, U., & Crapper McLachlan, D. R. (1981) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1981.tb04670.x · Cited by 78
Singer, D. S. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50624-4 · Cited by 57
Metadata
- Authors
- Maniatis, T., Jeffrey, A., & Van deSande, H.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1975-08-01
- Volume
- 14
- Issue
- 17
- Pages
- 3787-3794
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2152193561
- Cited by
- 1,121
- References
- 15
- FWCI
- 39.40
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
