Cited by 38DNA Repair MechanismsDNA and Nucleic Acid ChemistryBiochemical and Molecular Research

Base‐Excision Repair in Carrot Cells: Partial Purification and Characterization of Uracil‐DNA Glycosylase and Apurinic/Apyrimidinic Endodeox yribonuclease

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TALPAERT‐BORLÉ, Myriam, LIUZZI, Michel·1982·European Journal of Biochemistry

Abstract

Uracil‐DNA glycosylase and apurinic/apyrimidinic (AP) endodeoxyribonuclease hacc been purified from cultured carrot cells.The two enzymes, separated by affinity chromatography on Sepharosc‐poly(rU), were found to have properties similar to those of the homologous bacterial and mammalian enzymes. the action of AP endo‐ deoxyribonuclease on (dA)230· (dT, dU)230 partially depyrimidinated by uracil‐DNA glycosylase suggests that these two enzymes might act successively to initiate the repair of uracil‐containing DNA.

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  1. [10]10. Bio-Rad Instruction Manual, Bulletin 4407, October 1977. 346-352. 215-220. 1199-1209. Chem. 254,6387 -6391. 14321033, 1982, 3, Downloaded from https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1982.tb06611.x, Wiley Online Library on [07/04/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
  2. [14]14. Biof. Cheni. in the press.
  3. [17]17. M. Talpaert-Bode and M . Liuzzi, Laboratory of Biochemistry, Biology Group, D.G. X11, Ispra, Commission of The European Communities, Joint Research Centre, 1-21020 Ispra, Italy 14321033, 1982, 3, Downloaded from https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1982.tb06611.x, Wiley Online Library on [07/04/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License

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Authors
TALPAERT‐BORLÉ, M., & LIUZZI, M.
Affiliations
Joint Research Centre (IT)
Venue
European Journal of Biochemistry
Publisher
Wiley
Published
1982-06
Volume
124
Issue
3
Pages
435-440
ISSN
  • 0014-2956 (ISSN-L)
  • 1432-1033
OpenAlex
W1507294212
Cited by
38
References
18
Citations by year
2025: 1 · 2023: 1 · 2020: 2 · 2019: 1 · 2015: 1 · 2014: 1
FWCI
1.02
Type
journal article
Language
en
Open access
Locations
  • European Journal of Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
Joint Research Centre
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords
base excision repair, uracil-DNA glycosylase, AP endonuclease, carrot cells, DNA depurination, enzyme purification, DNA repair enzymes
MeSH terms
Deoxyribonucleases, DNA Repair, Endonucleases, Escherichia coli, Kinetics, N-Glycosyl Hydrolases, Plants, Time Factors, Escherichia coli Proteins, Deoxyribonuclease IV (Phage T4-Induced), DNA-(Apurinic or Apyrimidinic Site) Lyase, DNA Glycosylases, Uracil-DNA Glycosidase

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