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A Shuttle Mechanism for DNA‐Protein Interactions: The Regulation of Poly(ADP‐ribose) Polymerase
Open accessAbstract
Previously it had been shown that poly(ADP‐ribose) polymerase requires DNA for its activity and that this enzyme is auto‐poly(ADP‐ribosyl)ated. The studies reported here indicate that this self‐modification inhibits the enzyme and decreases its affinity for DNA, as shown by sucrose gradient density centrifugation. The coupling of poly(ADP‐ribose) polymerase with poly(ADP‐ribose) glycohydrolase reactivates the polymerase by degrading poly(ADP‐ribose) and restoring the polymerase‐DNA complex. The assay of polymerase in the presence of glycohydrolase was made possible by use of a double‐label assay involving release of 14 C‐labelled nicotinamide and the incorporation of 3 H‐labelled ADP‐ribose from NAD+. These results provide the basis for a shuttle mechanism in which the polymerase can be moved on and off DNA by the action of these two enzymes. Mg 2+ and histone HI appear to activate the polymerase by increasing the affinity of the polymerase for DNA.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
PARP inhibition: PARP1 and beyond
Rouleau, M., Patel, A., Hendzel, M. J., Kaufmann, S. H., & Poirier, G. G. (2010) · Nature Reviews Cancer · doi:10.1038/nrc2812 · Cited by 1,298
Klungland, A. (1997) · The EMBO Journal · doi:10.1093/emboj/16.11.3341 · Cited by 788
Hassa, P. (2008) · Frontiers in Bioscience · doi:10.2741/2909 · Cited by 584
Poly (ADP-ribose) polymerase, nitric oxide and cell death
Pieper, A. (1999) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(99)01292-4 · Cited by 517
Molecular and biochemical features of poly (ADP-ribose) metabolism
Lautier, D., Lagueux, J., Thibodeau, J., M�nard, L., & Poirier, G. G. (1993) · Molecular and Cellular Biochemistry · doi:10.1007/bf01076101 · Cited by 301
Metadata
- Authors
- ZAHRADKA, P., & EBISUZAKI, K.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1982-10
- Volume
- 127
- Issue
- 3
- Pages
- 579-585
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1565928793
- Cited by
- 182
- References
- 1
- FWCI
- 2.62
- Type
- journal article
- Language
- en
- Open access
- bronze
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- ZAHRADKA, P., & EBISUZAKI, K. (1982). A Shuttle Mechanism for DNA‐Protein Interactions: The Regulation of Poly(ADP‐ribose) Polymerase. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1982.tb06912.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
