The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Isolation of human‐p53‐specific monoclonal antibodies and their use in the studies of human p53 expression
Abstract
The isolation and construction of a complete human p53 cDNA and subsequent expression in monkey cells is described. A set of new anti‐(human p53) monoclonal antibodies has also been obtained and used to show the expression of the human p53 cDNA in cos‐1 cells. These antibodies enable the specific detection of human p53, which is synthesised in the presence of p53 from other species. Fusion proteins of p53 with β‐galactosidase were used firstly as antigen and secondly, in conjunction with competition assays, to localise the determinants recognised by the antibodies. At least two previously unrecognised epitopes are involved and two of the antibodies are human‐p53‐specific. The epitopes are denaturation‐resistant and the antibodies are, therefore, valuable for immunoblotting as well as immunoprecipitation and enzyme‐linked immunoassay. Transfection of plasmids containing complete human p53 cDNA into monkey (cos‐1) cells cause expression of human p53 recognised by the monoclonal antibodies. Control plasmids did not induce immunoreactive protein.
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Cites
Alitalo, K., Schwab, M., Lin, C. C., Varmus, H. E., & Bishop, J. M. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.6.1707 · Cited by 761
Cited by
Momand, J., Zambetti, G. P., Olson, D. C., George, D., & Levine, A. J. (1992) · Cell · doi:10.1016/0092-8674(92)90644-r · Cited by 3,113
P53 expression in breast cancer
Cattoretti, G., Rilke, F., Andreola, S., D'Amato, L., & Delia, D. (1988) · International Journal of Cancer · doi:10.1002/ijc.2910410204 · Cited by 601
Regulation of p53 function and stability by phosphorylation.
Ashcroft, M., Kubbutat, M. H. G., & Vousden, K. H. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.3.1751 · Cited by 452
Somatic mutations in the p53 tumor suppressor gene in rheumatoid arthritis synovium
Firestein, G. S., Echeverri, F., Yeo, M., Zvaifler, N. J., & Green, D. R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.20.10895 · Cited by 377
The XPB and XPD DNA helicases are components of the p53-mediated apoptosis pathway.
Wang, X. W., Vermeulen, W., Coursen, J. D., Gibson, M., Lupold, S. E., Forrester, K., Xu, G., Elmore, L., Yeh, H., Hoeijmakers, J. H., et al. (1996) · Genes & Development · doi:10.1101/gad.10.10.1219 · Cited by 313
Metadata
- Authors
- BANKS, L., MATLASHEWSKI, G., & CRAWFORD, L.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1986-09
- Volume
- 159
- Issue
- 3
- Pages
- 529-534
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2057397700
- Cited by
- 495
- References
- 1
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- BANKS, L., MATLASHEWSKI, G., & CRAWFORD, L. (1986). Isolation of human‐p53‐specific monoclonal antibodies and their use in the studies of human p53 expression. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1986.tb09919.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
