A Blue Non‐Heme Iron Protein from Desulfovibrio gigas
Abstract
A novel iron‐containing blue protein, named neelaredoxin, was isolated from the sulfate‐reducing bacterium Desulfovibrio gigas. It is a monomeric protein with a molecular mass of 15 kDa containing two iron atoms/molecule. The N‐terminal sequence of neelaredoxin has similarity to the second domain of desulfoferrodoxin, a protein purified from Desulfovibrio vulgaris Hildenborough. This finding supports the hypothesis that the gene coding for desulfoferrodoxin (rbo) might have arisen from a gene fusion [Brumlik, M. J., Leroy, G., Bruschi, M. & Voordouw, G. (1990) J. Bacteriol. 172, 7289–7292]. The visible spectrum exhibits a single band at 666 nm, responsible for the blue color of the protein, which is completely bleached upon reduction with sodium ascorbate. In the oxidized state the EPR spectrum is complex, exhibiting well‐resolved features at g = 7.6, 7.0, 5.9, and 5.8 which are assigned to two high‐spin (S= 5/2) mononuclear‐iron (III) centers with different rhombic distortions (E/D≈ 0.05 and ≈ 0.08). The two iron atoms contribute identically to the visible spectrum as judged from visible redox titrations, from which a reduction potential of + 190 mV was determined for both iron sites at pH 7.5. At high pH the visible and the EPR spectra become pH‐dependent with a p Ka above 9: the 666–nm band shifts to 590 nm and the EPR signals are converted into a signal with g max∼ 4.7. Neelaredoxin is readily reduced both by H 2/hydrogenase/cytochrome c 3 and by NADH/NADH–rubredoxin oxidoreductase.
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- Authors
- Chen, L., Sharma, P., Le Gall, J., Mariano, A. M., Teixeira, M., & Xavier, A. V.
- Affiliations
- University of Georgia (US)
- Universidade Nova de Lisboa (PT)
- Corresponding author
- Teixeira, Miguel
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1994-12
- Volume
- 226
- Issue
- 2
- Pages
- 613-618
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2022523144
- Cited by
- 77
- References
- 33
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2020: 2 · 2019: 2 · 2018: 2 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 1
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Keywords
- Desulfovibrio gigas, sulfate-reducing bacteria, redox potential, gene fusion, redox titration, visible absorption spectra
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Desulfovibrio, Electrochemistry, Electron Spin Resonance Spectroscopy, Iron, Metalloproteins, Molecular Sequence Data, Molecular Weight, Oxidation-Reduction, Spectrophotometry, Nonheme Iron Proteins
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