Purification and Characterization of Protein PB of Betaine Reductase and its Relationship to the Corresponding Proteins Glycine Reductase and Sarcosine Reductase from Eubacterium Acidaminophilum
Open accessAbstract
Simple complementation assay systems were developed for the substrate‐specific proteins P B of glycine reductase, sarcosine reductase, and betaine reductase, in which acetyl phosphate was detected as the product in all three cases. The betaine‐specific subunits of protein B (P B betaine) responsible for betaine reductase activity were purified to homogeneity from cells of Eubacterium acidaminophilum. The molecular masses of the two different subunits were 45 kDa and 48 kDa according to SDS/PAGE. The molecular mass of the native protein was about 200 kDa, indicating an α 2 β 2 structure. The glycine‐specific protein B (P B glycime) was partially purified and subunits of 47 kDa and 27 kDa were N‐terminally sequenced. The latter subunits cross‐reacted with antibodies raised against P B betaine and showed high sequence similarity to the 45‐kDa and 48‐kDa subunits of P B betaine, respectively. (2‐14 C]Glycine could be covalently coupled to the 47‐kDa subunit by treatment with borohydride. By the same procedure, [2‐14 C]sarcosine labeled a protein of the same size. Like the sarcosine reductase activity, this protein was not present in glycine‐grown cells, indicating its specific involvement in sarcosine metabolism. The labile viologen‐dependent formate dehydrogenase purified with the respective P B proteins and could be tentatively assigned to a 95‐kDa protein.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
There are data showing that adherence to a Mediterranean diet is more likely to result in lower levels of TMAO.
“there are also data showing that adherence to a Mediterranean diet is more likely to result in lower levels of TMAO222.”
From Dietary metabolism, the gut microbiome, and heart failure (2019) · cites this paper as [222]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Weber, K., & Osborn, M. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)94333-4 · Cited by 16,688 · see citing works
Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii
Voth, D. E., & Heinzen, R. A. (2007) · Cellular Microbiology · doi:10.1111/j.1462-5822.2007.00901.x · Cited by 11,773 · see citing works
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
Blum, H., Beier, H., & Gross, H. J. (1987) · Electrophoresis · doi:10.1002/elps.1150080203 · Cited by 4,150 · see citing works
Chamberlain, J. P. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90716-4 · Cited by 1,970 · see citing works
A SPECIFIC MICROMETHOD FOR THE DETERMINATION OF ACYL PHOSPHATES
Lipmann, F. A., & Lc, T. (1945) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)51298-4 · Cited by 1,422 · see citing works
Zindel, U., Freudenberg, W., Rieth, M., Andreesen, J. R., Schnell, J., & Widdel, F. (1988) · Archives of Microbiology · doi:10.1007/bf00407789 · Cited by 168 · see citing works
Self-citationPolyacrylamide Gel-electrophoresis across a Molecular Sieve Gradient
Margolis, J., & Kenrick, K. G. (1967) · Nature · doi:10.1038/2141334a0 · Cited by 130 · see citing works
Glycine metabolism in anaerobes
Andreesen, J. R. (1994) · Antonie van Leeuwenhoek · doi:10.1007/bf00871641 · Cited by 116 · see citing works
Self-citationHormann, K., & Andreesen, J. R. (1989) · Archives of Microbiology · doi:10.1007/bf00277541 · Cited by 83 · see citing works
Self-citationNaumann, E., Hippe, H., & Gottschalk, G. (1983) · Applied and Environmental Microbiology · doi:10.1128/aem.45.2.474-483.1983 · Cited by 82 · see citing works
Jollie, D. R., & Lipscomb, J. D. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54716-5 · Cited by 78 · see citing works
Recsei, P. A., Huynh, Q. K., & Snell, E. E. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.4.973 · Cited by 66 · see citing works
Tanaka, H., & Stadtman, T. C. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37938-3 · Cited by 60 · see citing works
Wagner, R., & Andreesen, J. R. (1977) · Archives of Microbiology · doi:10.1007/bf00446865 · Cited by 60 · see citing works
Self-citationFendrich, C., Hippe, H., & Gottschalk, G. (1990) · Archives of Microbiology · doi:10.1007/bf00423321 · Cited by 58 · see citing works
Hodgins, D. S., & Abeles, R. H. (1967) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99489-5 · Cited by 51 · see citing works
Arkowitz, R. A., & Abeles, R. H. (1991) · Biochemistry · doi:10.1021/bi00230a039 · Cited by 45 · see citing works
Dietrichs, D., Meyer, M., Schmidt, B., & Andreesen, J. R. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.4.2088-2095.1990 · Cited by 42 · see citing works
Self-citationArkowitz, R. A., & Abeles, R. H. (1989) · Biochemistry · doi:10.1021/bi00437a019 · Cited by 41 · see citing works
Dietrichs, D., Meyer, M., Rieth, M., & Andreesen, J. R. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.19.5983-5991.1991 · Cited by 35 · see citing works
Self-citationFreudenberg, W., Dietrichs, D., Lebertz, H., et al. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.3.1346-1354.1989 · Cited by 34 · see citing works
Freudenberg, W., & Andreesen, J. R. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.4.2209-2215.1989 · Cited by 33 · see citing works
Self-citationChromatography of proteins on diethylaminoethyl-cellulose in concentrated ammonium sulfate
Mayhew, S. G., & Howell, L. G. (1971) · Analytical Biochemistry · doi:10.1016/0003-2697(71)90166-7 · Cited by 30 · see citing works
Components of glycine reductase from Eubacterium acidaminophilum
Lübbers, M., & Andreesen, J. R. (1993) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1993.tb18307.x · Cited by 26 · see citing works
Self-citationAcetate via Glycine: A Different Form of Acetogenesis
Andreesen, J. R. (1994) · Springer eBooks · doi:10.1007/978-1-4615-1777-1_23 · Cited by 26 · see citing works
Self-citationSCHRÄDER, T., & Andreesen, J. R. (1992) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1992.tb16903.x · Cited by 25 · see citing works
Self-citationMeyer, M., Dietrichs, D., Schmidt, B., & Andreesen, J. R. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.4.1509-1513.1991 · Cited by 18 · see citing works
Self-citationA pyruvate-containing peptide of proline reductase in Clostridium sticklandii
Seto, B. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30419-2 · Cited by 17 · see citing works
Flagged first, then by citations
Research works printed but not linked: 4
- Granderath, K. (1988) Physiologische und enzymatische Unter- suchungen zum Abbau von Aminosiuren und organischen Siuren durch Eubacterium aci(kEnzinoplzilum, Diploma thesis, University of Gijttingen.
- Friedebold, J. (1994) Das Formiat-oxidierende Enzymsystem in Alcnligenes eutrojihus: Biochemische Charakterisierung der loslichen Formiat-Dehydrogenase, Ph.D. Thesis, University of Gottingen.
- Bliidorn, B. (1993) Isolierung eines 63 kDa-Proteins und vergleichende Charakterisierungen mit der Acetat-Kinase aus Eubacleriuin cici~r/nikr)phiIuln. Diploma thesis. University of Gottingen.
- Rieth, M. (1987) Untersuchungen m r selenabhangigen Glycin-Re- duktase aus Euhacterium acidaminophilum, Ph.D. Thesis, Univer- sity of Gottingen.
Cited by
Sorted by citations
Dietary metabolism, the gut microbiome, and heart failure
Tang, W. H. W., Li, D. Y., & Hazen, S. L. (2019) · Nature Reviews Cardiology · doi:10.1038/s41569-018-0108-7 · Cited by 789 · see citing works
Andreesen, J. R. (2004) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2004.08.002 · Cited by 86 · see citing works
Self-citationWagner, M., Sonntag, D., Grimm, R., Pich, A., Eckerskorn, C., Söhling, B., & Andreesen, J. R. (1999) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.1999.00107.x · Cited by 74 · see citing works
Self-citation
- Authors
- Meyer, M., Granderath, K., & Andreesen, J. R.
- Affiliations
- University of Göttingen (DE)
- Martin Luther University Halle-Wittenberg (DE)
- Corresponding author
- Andreesen, Jan R.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1995-11
- Volume
- 234
- Issue
- 1
- Pages
- 184-191
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2143418808
- Cited by
- 51
- References
- 30
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 3 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 1 · 2012: 2
- FWCI
- 0.62
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Keywords
- glycine reductase, protein B, protein purification, complementation assay, acetyl phosphate, glycine metabolism, protein subunit structure, N-terminal sequence
- MeSH terms
- Amino Acid Oxidoreductases, Amino Acid Sequence, Betaine, Electrophoresis, Polyacrylamide Gel, Eubacterium, Formate Dehydrogenases, Glycine, Molecular Sequence Data, Multienzyme Complexes, Sarcosine, Substrate Specificity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
