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Distinct Repression of Translation by Wortmannin and Rapamycin

Pedersen, Susanne, Celis, Julio E., Nielsen, Jacob, Christiansen, Jan, Nielsen, Finn C.·1997·European Journal of Biochemistry
DOI 10.1111/j.1432-1033.1997.00449.x
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Abstract

The role of phosphatidylinositol 3‐kinase and FK506‐binding protein · rapamycin‐associated protein (FRAP) in translational control has been examined by treating RD‐rhabdomyosarcoma cells with wortmannin and rapamycin and studying the effects on cell‐growth, translation initiation, and protein synthesis. Whereas wortmannin and rapamycin exhibit subtle effects on global translation, examination of individual mRNAs in sucrose gradients and of individual proteins in two‐dimensional polyacrylamide gels reveals that wortmannin and rapamycin exhibit distinct effects on the translation of individual mRNAs. Wortmannin represses the synthesis of a third of cellular proteins, whereas rapamycin affects a subset of these proteins. Since ribosomal protein S6 was rapidly dephosphorylated following wortmannin and rapamycin treatment, and the phosphorylation status of the eukaryotic initiation factor 4E was unchanged, our data imply that the p70 signalling pathway has at least one branch‐point upstream of FRAP leading to an additional route of translational control.

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Metadata

Authors
Pedersen, S., Celis, J. E., Nielsen, J., Christiansen, J., & Nielsen, F. C.
Venue
European Journal of Biochemistry
Publisher
Wiley
Published
1997-07
Volume
247
Issue
1
Pages
449-456
ISSN
  • 0014-2956
  • 1432-1033
DOI
10.1111/j.1432-1033.1997.00449.x
Cited by
36
References
0
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 2572 d)

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