Body distribution of infused serotonin in rats
Open accessAbstract
1. Our goal was to investigate the body distribution of serotonin (5-hydroxytryptamine; 5-HT) in rats infused with 5-HT (25 microg/kg per min) for 7 days and the contribution of the 5-HT transporter (SERT) for 5-HT uptake into the tissues. 2. Mini-osmotic pumps containing 5-HT or vehicle were implanted in rats knocked out for SERT (SERT-KO) or in wild-type (WT) rats. On the 8th day, tissues were harvested for measurements of 5-HT by high-performance liquid chromatography (HPLC). The 5-HT metabolite 5-hydroxyindole acetic acid (5-HIAA) was also measured by HPLC, because an increase in 5-HIAA in tissues from rats receiving 5-HT reflects 5-HT uptake followed by metabolism. 3. In WT rats infused with 5-HT, an increase in 5-HT or 5-HIAA was observed in the heart, pancreas, thyroid, adrenal gland, kidney, seminal vesicle, bladder, prostate, liver, oesophagus, stomach, femur, trachea, lung and spleen compared with vehicle-infused rats. An increase in 5-HT and 5-HIAA was not observed in aorta, vena cava and jejunum. In tissues from SERT-KO rats infused with 5-HT, the content of 5-HT or 5-HIAA was decreased in most of the tissues studied compared with 5-HT-infused WT rats. Although 5-HT uptake in the kidney, seminal vesicle, prostate, jejunum and trachea is SERT dependent, it is SERT independent in the pancreas. The remaining tissues display SERT-dependent and -independent mechanisms for 5-HT uptake. 4. Altogether, tissues from different systems, such as the cardiovascular, endocrine, genitourinary and gastrointestinal, accumulate 5-HT mainly via SERT and, thus, these systems are potential targets for drugs that interfere with 5-HT homeostasis.
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Adrenal chromaffin cells recycle 5-HT through serotonin transporter (SERT).
“It has been shown that adrenal chromaffin cells recycle 5-HT through serotonin transporter (SERT) (Brindley et al., 2016; Linder et al., 2009).”
From Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells (2021) · cites this paper as Linder et al., 2009
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Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells
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- Authors
- Linder, A. E., Beggs, K. M., Burnett, R. J., & Watts, S. W.
- Affiliations
- Michigan State University (US)
- Corresponding author
- Linder, A Elizabeth
- ORCID
- Watts, Stephanie W
- Venue
- Clinical and Experimental Pharmacology and Physiology
- Publisher
- Wiley
- Published
- 2009-04-27
- Volume
- 36
- Issue
- 5-6
- Pages
- 599-601
- ISSN
- 0305-1870 (ISSN-L)
- 1440-1681
- OpenAlex
- W2001909401
- Cited by
- 28
- References
- 17
- Citations by year
- 2025: 1 · 2023: 4 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 2 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Clinical and Experimental Pharmacology and Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacogenetics and Drug Metabolism
- Keywords
- serotonin, tissue distribution, serotonin transporter, 5-hydroxyindoleacetic acid, serotonin uptake, tissue accumulation, serotonin homeostasis
- MeSH terms
- Animals, Biological Availability, Drug Evaluation, Preclinical, Infusion Pumps, Male, Serotonin, Tissue Distribution, Rats, Wistar, Serotonin Plasma Membrane Transport Proteins, Rats, Rats, Transgenic
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