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Cited by 14Animal Virus Infections StudiesVirology and Viral DiseasesInfectious Encephalopathies and Encephalitis

Cytopathy of an infiltrating monocyte lineage during the early phase of infection with murinecoronavirus in the brain: Cytopathy of infiltrating monocytes

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Takatsuki, Hanae, Taguchi, Fumihiro, Nomura, Risa, Kashiwazaki, Hiromi, Watanabe, Mariko, Ikehara, Yuzuru, Watanabe, Rihito·2009·Neuropathology
DOI 10.1111/j.1440-1789.2009.01082.xPMC PMC7194124
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Abstract

Viral spread during the early stages after infection was compared between a highly neurovirulent mouse hepatitis virus (MHV), JHMV cl-2 strain (cl-2), and its low-virulent mutant, soluble-receptor-resistant (srr)7. The infection of cells with srr7 (soluble-receptor-resistant mutant 7) is dependent on a known MHV receptor (MHVR), carcinoembryonic cell adhesion molecule 1a, whereas cl-2 shows MHVR-independent infection. Initial viral antigens were detected between 12 and 24 h post-inoculation (p.i) in the infiltrating cells that appeared in the subarachnoidal space of mouse brains infected with viruses. There were no significant differences in the intensity or spread of viral antigens in the inflammatory cells between the two viruses. However, 48 h after infection with cl-2, viral antigen-positive cells in the grey matter with the shape of neurons, which do not express MHVR, were detected, while srr7 infection was observed primarily in the white matter. Some of the viral antigen-positive inflammatory cells found in the subarachnoidal space during the early phase of infection reacted with anti-F4/80 or anti-CD11b monoclonal antibodies. Syncytial giant cells (SGCs) expressing viral and CD11b antigens were also detected among these inflammatory cells. These antigen-positive cells appeared in the subarachnoidal space prior to viral antigen spread into the brain parenchyma, indicating that viral encephalitis starts with the infection of infiltrating monocytes which express MHVR. Furthermore, the observation indicates that viral infection has cytopathic effects on the monocyte lineage, which plays a critical role in innate immunity, leading to the rapid spread of viruses during the early stage of infection.

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Crossref · 10.1111/j.1440-1789.2009.01082.x

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  • During murine CNS infections, including mouse hepatitis virus and LCMV Armstrong, there is an influx into the brain and subarachnoid space of CD8+ T cells, monocytes and neutrophils into the meninges quickly after infection.

    “during murine CNS infections, including mouse hepatitis virus, a murine coronavirus (strains Srr7, cl-2, and A59), the Armstrong strain of lymphocytic choriomeningitis virus (LCMV), all show there is an influx into the brain and subarachnoid space of CD8+ T cells, monocytes and neutrophils into the meninges quickly after infection”

    From Living on the Edge of the CNS: Meninges Cell Diversity in Health and Disease (2021) · cites this paper as Takatsuki et al., 2009

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Authors
Takatsuki, H., Taguchi, F., Nomura, R., Kashiwazaki, H., Watanabe, M., Ikehara, Y., & Watanabe, R.
Affiliations
  • Soka University (JP)
  • Nippon Veterinary and Life Science University (JP)
  • Tokyo University of Agriculture and Technology (JP)
Corresponding author
Watanabe, Rihito
ORCID
Venue
Neuropathology
Publisher
Wiley
Published
2009-12-28
Volume
30
Issue
4
Pages
361-371
ISSN
  • 0919-6544 (ISSN-L)
  • 1440-1789
DOI
10.1111/j.1440-1789.2009.01082.x
PMC
PMC7194124
OpenAlex
W1859244832
Cited by
14
References
45
Citations by year
2022: 1 · 2021: 1 · 2017: 3 · 2016: 2 · 2014: 3
FWCI
2.35
Type
journal article
Language
en
Open access
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 2073 d)
Locations
  • Neuropathology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Education, Culture, Sports, Science and Technology
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Health Sciences › Medicine › Infectious Diseases › Infectious Encephalopathies and Encephalitis
Keywords
mouse hepatitis virus, viral receptor, monocyte lineage, cytopathic effect, viral encephalitis, brain infection, subarachnoid space, innate immunity, viral spread, white matter, grey matter
MeSH terms
Animals, Antigens, Viral, Brain, Coronaviridae Infections, Fluorescent Antibody Technique, Glycoproteins, Murine hepatitis virus, Immunohistochemistry, Mice, Inbred BALB C, Monocytes, Cell Adhesion Molecules, Cell Lineage, Mice

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