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Cited by 19Corneal surgery and disordersCorneal Surgery and TreatmentsOcular Surface and Contact Lens

An explanation for the central to peripheral thickness variation in the mouse cornea

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Henriksson, Johanna Tukler, Bron, Anthony J, Bergmanson, Jan PG·2012·Clinical & Experimental Ophthalmology
DOI 10.1111/j.1442-9071.2011.02652.xPMC PMC4120677
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Abstract

Background: The mouse corneal stroma varies in thickness across its diameter. The purpose of the present study was to explain this variation and to advance our understanding of stromal lamellar architecture in the mammalian cornea. Methods: Eight C57BL/6 mice were killed, eyes enucleated, immersed in 2% glutaraldehyde fixative, processed and sectioned transversely for light and transmission electron microscopy. Transmission electron micrographs were assembled into montages and printed at 5000× magnification and used for lamellar counts and thickness assessments. Results: The mouse cornea had an average of 49.8 ± 2.4 lamellae centrally averaging 2.1 µm in thickness versus 35.5 ± 3.0 lamellae, averaging 1.9 µm in thickness peripherally. The central to peripheral decrease in number lamellae and lamellar thickness measured utilizing the transmission electron microscope was statistically significant (P < 0.005). Conclusions: This study demonstrated that the thickness difference between the thicker central and thinner peripheral mouse cornea is explained primarily by the number of lamellae present and that the peripheral lamellar dropout occurred in the anterior 2/3 of stroma. The decreased lamellar count towards the periphery suggested that not all lamellae cross the cornea limbus to limbus. These findings may be relevant to the thickness variation of the human cornea.

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  • It seems likely that alterations in tissue thickness from central cornea to limbus in human corneas are due to an increase in the amount of collagen in the peripheral stroma.

    “although water distribution from central cornea to limbus is not known for human corneas, it seems likely that alterations in tissue thickness are due to an increase in the amount of collagen in the peripheral stroma (Aghamohammadzadeh et al., 2004, Boote et al., 2011, Henriksson et al., 2012).”

    From Corneal structure and transparency (2015) · cites this paper as Henriksson et al., 2012

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Research works printed but not linked: 44

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Authors
Henriksson, J. T., Bron, A. J., & Bergmanson, J. P.
Affiliations
  • University of Houston (US)
  • University of Oxford (GB)
Corresponding author
Bergmanson, Jan PG
ORCID
Bergmanson, Jan PG
Venue
Clinical & Experimental Ophthalmology
Publisher
Wiley
Published
2011-09-19
Volume
40
Issue
2
Pages
174-181
ISSN
  • 1442-6404 (ISSN-L)
  • 1442-9071
DOI
10.1111/j.1442-9071.2011.02652.x
PMC
PMC4120677
OpenAlex
W2111000486
Cited by
19
References
0
Citations by year
2026: 1 · 2025: 3 · 2024: 3 · 2023: 3 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2015: 2 · 2013: 1 · 2012: 1
FWCI
0.47
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Clinical and Experimental Ophthalmology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • University of Houston
  • National Eye Institute (P30EY007551)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal surgery and disorders
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal Surgery and Treatments
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ocular Surface and Contact Lens
Keywords
corneal stroma, corneal limbus, mouse cornea, human cornea
MeSH terms
Animals, Body Weights and Measures, Collagen, Cornea, Corneal Stroma, Cytoplasm, Mice, Inbred C57BL, Microscopy, Electron, Transmission, Mice, Corneal Keratocytes

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