The DOI matches its Crossref record; 70 of the 70 references it cites were found in a registry or an index.
Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques
Open accessAbstract
Summary: Fungi fulfil a range of important ecological functions, yet current understanding of fungal biodiversity in soil is limited. Direct DNA extraction from soil, coupled with polymerase chain reaction amplification and community profiling techniques, has proved successful in investigations of bacterial ecology and shows great promise for elucidating the taxonomic and functional characteristics of soil fungal communities. These community profiling techniques include denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), single‐strand conformation polymorphism (SSCP), terminal restriction fragment length polymorphism (T‐RFLP), amplified rDNA restriction analysis (ARDRA), amplified ribosomal intergenic spacer analysis (ARISA) and cloning, and are generally coupled with DNA sequencing. The techniques and their potential limitations are discussed, along with recent advances that have been made possible through their application in soil fungal ecology. It is unlikely that a single approach will be universally applicable for assessing fungal diversity in all soils or circumstances. However, judicious selection of the methodology, keeping the experimental aims in mind, and the exploitation of emerging technologies will undoubtedly increase our understanding of soil fungal communities in the future.
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Cites
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Community structure and metabolism through reconstruction of microbial genomes from the environment
Tyson, G. W., Chapman, J., Hugenholtz, P., Allen, E. E., Ram, R. J., Richardson, P. M., Solovyev, V. V., Rubin, E. M., Rokhsar, D. S., & Banfield, J. F. (2004) · Nature · doi:10.1038/nature02340 · Cited by 2,417
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Cited by
Environmental DNA – An emerging tool in conservation for monitoring past and present biodiversity
Thomsen, P. F., & Willerslev, E. (2015) · Biological Conservation · doi:10.1016/j.biocon.2014.11.019 · Cited by 2,082
Fernandez, C. W., & Kennedy, P. G. (2016) · New Phytologist · doi:10.1111/nph.13648 · Cited by 532
A practical guide to the oral microbiome and its relation to health and disease
Krishnan, K., Chen, T., & Paster, B. (2017) · Oral Diseases · doi:10.1111/odi.12509 · Cited by 347
“…techniques such as denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) separated DNA of the same size, which in turn could be sequenced for identification purposes (Anderson & Cairney, 2004, Deng et al., 2008, Nishigaki et al., 2000).”
MethodLiu, J., Sui, Y., Yu, Z., Shi, Y., Chu, H., Jin, J., Liu, X., & Wang, G. (2015) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2015.01.009 · Cited by 310
Allison, S. D., Hanson, C. A., & Treseder, K. K. (2007) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2007.02.001 · Cited by 305
Metadata
- Authors
- Anderson, I. C., & Cairney, J. W. G.
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2004-06-28
- Volume
- 6
- Issue
- 8
- Pages
- 769-779
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W2152134501
- Cited by
- 558
- References
- 70
- FWCI
- 34.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Anderson, I. C., & Cairney, J. W. G. (2004). Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques. Environmental Microbiology. https://doi.org/10.1111/j.1462-2920.2004.00675.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
