The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Culturing captures members of the soil rare biosphere
Open accessAbstract
Summary: The ecological significance of rare microorganisms within microbial communities remains an important, unanswered question. Microorganisms of extremely low abundance (the ‘rare biosphere’) are believed to be largely inaccessible and unknown. To understand the structure of complex environmental microbial communities, including the representation of rare and prevalent community members, we coupled traditional cultivation with pyrosequencing. We compared cultured and uncultured bacterial members of the same agricultural soil, including eight locations within one apple orchard and four time points. Our analysis revealed that soil bacteria captured by culturing were in very low abundance or absent in the culture‐independent community, demonstrating unexpected accessibility of the rare biosphere by culturing.
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Citations
Cites
Prokaryotes: The unseen majority
Whitman, W. B., Coleman, D. C., & Wiebe, W. J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.6578 · Cited by 4,871 · cited 1×
“Microorganisms are the most abundant organisms on Earth and represent an unfathomably high level of diversity (Whitman et al., 1998; Rappé and Giovannoni, 2003; Schloss and Handelsman, 2007).”
BackgroundIroning out the wrinkles in the rare biosphere through improved OTU clustering
Huse, S. M., Welch, D. M., Morrison, H. G., & Sogin, M. L. (2010) · Environmental Microbiology · doi:10.1111/j.1462-2920.2010.02193.x · Cited by 1,346
Cited by
High sensitivity of 454 pyrosequencing for detection of rare species in aquatic communities
Zhan, A., Hulák, M., Sylvester, F., Huang, X., Adebayo, A. A., Abbott, C. L., Adamowicz, S. J., Heath, D. D., Cristescu, M. E., & MacIsaac, H. J. (2013) · Methods in Ecology and Evolution · doi:10.1111/2041-210x.12037 · Cited by 292
Thijs, S., Sillen, W., Rineau, F., Weyens, N., & Vangronsveld, J. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00341 · Cited by 265
Diverse alkane hydroxylase genes in microorganisms and environments
Nie, Y., Chi, C.-Q., Fang, H., Liang, J.-L., Lu, S.-L., Lai, G.-L., Tang, Y.-Q., & Wu, X.-L. (2014) · Scientific Reports · doi:10.1038/srep04968 · Cited by 261
Metadata
- Authors
- Shade, A., Hogan, C. S., Klimowicz, A. K., Linske, M., McManus, P. S., & Handelsman, J.
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2012-07-13
- Volume
- 14
- Issue
- 9
- Pages
- 2247-2252
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W1606930879
- Cited by
- 210
- References
- 2
- FWCI
- 15.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Shade, A., Hogan, C. S., Klimowicz, A. K., Linske, M., McManus, P. S., & Handelsman, J. (2012). Culturing captures members of the soil rare biosphere. Environmental Microbiology. https://doi.org/10.1111/j.1462-2920.2012.02817.x
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