The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells
Open accessAbstract
Gram-negative bacterial peptidoglycan is specifically recognized by the host intracellular sensor NOD1, resulting in the generation of innate immune responses. Although epithelial cells are normally refractory to external stimulation with peptidoglycan, these cells have been shown to respond in a NOD1-dependent manner to Gram-negative pathogens that can either invade or secrete factors into host cells. In the present work, we report that Gram-negative bacteria can deliver peptidoglycan to cytosolic NOD1 in host cells via a novel mechanism involving outer membrane vesicles (OMVs). We purified OMVs from the Gram-negative mucosal pathogens: Helicobacter pylori, Pseudomonas aeruginosa and Neisseria gonorrhoea and demonstrated that these peptidoglycan containing OMVs upregulated NF-kappaB and NOD1-dependent responses in vitro. These OMVs entered epithelial cells through lipid rafts thereby inducing NOD1-dependent responses in vitro. Moreover, OMVs delivered intragastrically to mice-induced innate and adaptive immune responses via a NOD1-dependent but TLR-independent mechanism. Collectively, our findings identify OMVs as a generalized mechanism whereby Gram-negative bacteria deliver peptidoglycan to cytosolic NOD1. We propose that OMVs released by bacteria in vivo may promote inflammation and pathology in infected hosts.
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Cites
Nod1 Detects a Unique Muropeptide from Gram-Negative Bacterial Peptidoglycan
Girardin, S. E., Boneca, I. G., Carneiro, L. A. M., Antignac, A., Jéhanno, M., Viala, J., Tedin, K., Taha, M.-K., Labigne, A., Zäthringer, U., et al. (2003) · Science · doi:10.1126/science.1084677 · Cited by 1,577
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Fitzgerald, K. A., Palsson-McDermott, E. M., Bowie, A. G., Jefferies, C. A., Mansell, A. S., Brady, G., Brint, E., Dunne, A., Gray, P., Harte, M. T., et al. (2001) · Nature · doi:10.1038/35092578 · Cited by 1,256
Nod1 responds to peptidoglycan delivered by the Helicobacter pylori cag pathogenicity island
Viala, J., Chaput, C., Boneca, I. G., Cardona, A., Girardin, S. E., Moran, A. P., Athman, R., Mémet, S., Huerre, M. R., Coyle, A. J., et al. (2004) · Nature Immunology · doi:10.1038/ni1131 · Cited by 1,226
Self-citationBacterial outer membrane vesicles and the host–pathogen interaction
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A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strain
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Toll-like receptors and cancer
Rakoff-Nahoum, S., & Medzhitov, R. (2009) · Nature Reviews Cancer · doi:10.1038/nrc2541 · Cited by 925
Nod-like proteins in immunity, inflammation and disease
Fritz, J. H., Ferrero, R. L., Philpott, D. J., & Girardin, S. E. (2006) · Nature Immunology · doi:10.1038/ni1412 · Cited by 892
Self-citationThe Biogenesis and Functions of Exosomes
Stoorvogel, W., Kleijmeer, M. J., Geuze, H. J., & Raposo, G. (2002) · Traffic · doi:10.1034/j.1600-0854.2002.30502.x · Cited by 847
CARD4/Nod1 mediates NF‐κB and JNK activation by invasive Shigella flexneri
Girardin, S. E., Tournebize, R., Mavris, M., Page, A., Li, X., Stark, G. R., Bertin, J., DiStefano, P. S., Yaniv, M., Sansonetti, P. J., et al. (2001) · EMBO reports · doi:10.1093/embo-reports/kve155 · Cited by 609
Self-citationHuman Nod1 Confers Responsiveness to Bacterial Lipopolysaccharides
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Cited by
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Helicobacter pylori: gastric cancer and beyond
Polk, D. B., & Peek, R. M. (2010) · Nature Reviews Cancer · doi:10.1038/nrc2857 · Cited by 1,158
Immune modulation by bacterial outer membrane vesicles
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Self-citationThe Human Microbiota in Health and Disease
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Metadata
- Authors
- Kaparakis, M., Turnbull, L., Carneiro, L., Firth, S., Coleman, H. A., Parkington, H. C., Le Bourhis, L., Karrar, A., Viala, J., Mak, J., Hutton, M. L., Davies, J. K., Crack, P. J., Hertzog, P. J., Philpott, D. J., Girardin, S. E., Whitchurch, C. B., & Ferrero, R. L.
- Venue
- Cellular Microbiology
- Publisher
- Hindawi Limited
- Published
- 2010-03
- Volume
- 12
- Issue
- 3
- Pages
- 372-385
- ISSN
- 1462-5814 (ISSN-L)
- 1462-5822
- OpenAlex
- W2149980799
- Cited by
- 451
- References
- 43
- FWCI
- 7.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kaparakis, M., Turnbull, L., Carneiro, L., Firth, S., Coleman, H. A., Parkington, H. C., Le Bourhis, L., Karrar, A., Viala, J., Mak, J., Hutton, M. L., Davies, J. K., Crack, P. J., Hertzog, P. J., Philpott, D. J., Girardin, S. E., Whitchurch, C. B., & Ferrero, R. L. (2010). Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells. Cellular Microbiology. https://doi.org/10.1111/j.1462-5822.2009.01404.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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