The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Coordinated loading of IRG resistance GTPases on to the Toxoplasma gondii parasitophorous vacuole: Coordinated loading of IRGs on to the T. gondii vacuole
Open accessAbstract
The immunity-related GTPases (IRGs) constitute an interferon-induced intracellular resistance mechanism in mice against Toxoplasma gondii. IRG proteins accumulate on the parasitophorous vacuole membrane (PVM), leading to its disruption and to death of the parasite. How IRGs target the PVM is unknown. We show that accumulation of IRGs on the PVM begins minutes after parasite invasion and increases for about 1 h. Targeting occurs independently of several signalling pathways and the microtubule network, suggesting that IRG transport is diffusion-driven. The intensity of IRG accumulation on the PVM, however, is reduced in absence of the autophagy regulator, Atg5. In wild-type cells IRG proteins accumulate cooperatively on PVMs in a definite order reflecting a temporal hierarchy, with Irgb6 and Irgb10 apparently acting as pioneers. Loading of IRG proteins onto the vacuoles of virulent Toxoplasma strains is attenuated and the two pioneer IRGs are the most affected. The polymorphic rhoptry kinases, ROP16, ROP18 and the catalytically inactive proteins, ROP5A-D, are not individually responsible for this effect. Thus IRG proteins protect mice against avirulent strains of Toxoplasma but fail against virulent strains. The complex cooperative behaviour of IRG proteins in resisting Toxoplasma may hint at undiscovered complexity also in virulence mechanisms.
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Cites
The role of autophagy during the early neonatal starvation period
Kuma, A., Hatano, M., Matsui, M., Yamamoto, A., Nakaya, H., Yoshimori, T., Ohsumi, Y., Tokuhisa, T., & Mizushima, N. (2004) · Nature · doi:10.1038/nature03029 · Cited by 2,865
Gutierrez, M. G., Master, S. S., Singh, S. B., Taylor, G. A., Colombo, M. I., & Deretic, V. (2004) · Cell · doi:10.1016/j.cell.2004.11.038 · Cited by 2,252
The Atg12-Atg5 Conjugate Has a Novel E3-like Activity for Protein Lipidation in Autophagy
Hanada, T., Noda, N. N., Satomi, Y., Ichimura, Y., Fujioka, Y., Takao, T., Inagaki, F., & Ohsumi, Y. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.c700195200 · Cited by 1,123 · cited 1×
“Which if any of the increasing range of activities attributed to Atg5 (Hanada et al., 2007; Geng and Klionsky, 2008) could be responsible for this activity is unclear.”
BackgroundImmune Control of Tuberculosis by IFN-γ-Inducible LRG-47
MacMicking, J. D., Taylor, G. A., & McKinney, J. D. (2003) · Science · doi:10.1126/science.1088063 · Cited by 685 · cited 2×
“Because Irgm1 has been shown to relocate to phagocytic cups and phagosomes during particle uptake (MacMicking et al., 2003; Martens et al., 2004) it has been proposed that this IRG protein can implement a direct cell-autonomous attack on phagocytosed pathogens (MacMicking et al., 2003).”
Background
Cited by
Autophagy in immunity and inflammation
Levine, B., Mizushima, N., & Virgin, H. W. (2011) · Nature · doi:10.1038/nature09782 · Cited by 3,289
Interferon-inducible effector mechanisms in cell-autonomous immunity
MacMicking, J. D. (2012) · Nature Reviews Immunology · doi:10.1038/nri3210 · Cited by 579
Innate immunity to Toxoplasma gondii infection
Yarovinsky, F. (2014) · Nature Reviews Immunology · doi:10.1038/nri3598 · Cited by 462
Toxoplasma Effectors Targeting Host Signaling and Transcription
Hakimi, M.-A., Olias, P., & Sibley, L. D. (2017) · Clinical Microbiology Reviews · doi:10.1128/cmr.00005-17 · Cited by 339
IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes
Man, S. M., Karki, R., Sasai, M., Place, D. E., Kesavardhana, S., Temirov, J., Frase, S., Zhu, Q., Malireddi, R. K. S., Kuriakose, T., et al. (2016) · Cell · doi:10.1016/j.cell.2016.09.012 · Cited by 312
Metadata
- Authors
- Khaminets, A., Hunn, J. P., Könen-Waisman, S., Zhao, Y. O., Preukschat, D., Coers, J., Boyle, J. P., Ong, Y.-C., Boothroyd, J. C., Reichmann, G., & Howard, J. C.
- Venue
- Cellular Microbiology
- Publisher
- Hindawi Limited
- Published
- 2010-01-26
- Volume
- 12
- Issue
- 7
- Pages
- 939-961
- ISSN
- 1462-5814 (ISSN-L)
- 1462-5822
- OpenAlex
- W1571840817
- Cited by
- 204
- References
- 4
- FWCI
- 13.31
- Type
- journal article
- Language
- en
- Open access
- green
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- Khaminets, A., Hunn, J. P., Könen-Waisman, S., Zhao, Y. O., Preukschat, D., Coers, J., Boyle, J. P., Ong, Y.-C., Boothroyd, J. C., Reichmann, G., & Howard, J. C. (2010). Coordinated loading of IRG resistance GTPases on to the Toxoplasma gondii parasitophorous vacuole: Coordinated loading of IRGs on to the T. gondii vacuole. Cellular Microbiology. https://doi.org/10.1111/j.1462-5822.2010.01443.x
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