Evidence that mitochondria buffer physiological Ca2+ loads in lizard motor nerve terminals

Abstract

Changes in cytosolic and mitochondrial [Ca 2+] produced by brief trains of action potentials were measured in motor nerve terminals using a rapidly scanning confocal microscope. Cytosolic [Ca 2+] was measured using ionophoretically injected Oregon Green BAPTA 5N (OG‐5N). Mitochondrial [Ca 2+] was measured using rhod‐2, bath loaded as dihydrorhod‐2. In response to 100‐250 stimuli at 25‐100 Hz the average cytosolic [Ca 2+] showed an initial rapid increase followed by a much slower rate of increase. Mitochondrial [Ca 2+] showed no detectable increase during the first fifteen to twenty stimuli, but after this initial delay also showed an initially rapid rise followed by a slower rate of increase. The onset of the increase in mitochondrial [Ca 2+] coincided with the slowing of the rate of rise of cytosolic [Ca 2+]. The peak levels of cytosolic and mitochondrial [Ca 2+] both increased with increasing frequencies of stimulation. When stimulation terminated, the initial rate of decay of cytosolic [Ca 2+] was much more rapid than that of mitochondrial [Ca 2+]. After addition of carbonyl cyanide m‐chlorophenyl hydrazone (CCCP, 1‐2 μ m) to dissipate the proton electrochemical gradient across the mitochondrial membrane, cytosolic [Ca 2+] rose rapidly throughout the stimulus train, reaching levels much higher than normal. CCCP inhibited the increase in mitochondrial [Ca 2+]. These results suggest that mitochondrial uptake of Ca 2+ contributes importantly to buffering presynaptic cytosolic [Ca 2+] during normal neuromuscular transmission.