Mitochondrial Involvement in Post-Tetanic Potentiation of Synaptic Transmission
Open accessAbstract
Posttetanic potentiation (PTP) is an essential aspect of synaptic transmission that arises from a persistent presynaptic [Ca2+]i following tetanic stimulation. At crayfish neuromuscular junctions, several inhibitors of mitochondrial Ca2+ uptake and release (tetraphenylphosphonium or TPP+, carbonyl cyanide m-chlorophenylhydrazone or CCCP, and ruthenium red) blocked PTP and the persistence of presynaptic residual [Ca2+]i, while endoplasmic reticulum (ER) Ca2+ pump inhibitors and release channel activators (thapsigargin, 2,5-di-(tert-butyl)-1,4-benzohydroquinone or BHQ, and caffeine) had no effects. PTP apparently results from the slow efflux of tetanically accumulated mitochondrial Ca2+.
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A part of glutamate can be taken up by mitochondria, where it is metabolized to 2-oxoglutarate, contributing to ATP synthesis.
“A part of the glutamate can be taken up by the mitochondria, where it is metabolized to 2-oxoglutarate further contributing to the synthesis of ATP (Tang and Zucker, 1997; Verstreken et al., 2005), thereby removing it from the glutamine–glutamate cycle and reducing its toxicity (Vaglio-Garro et al., 2024b).”
From Toxic Concentrations of Glutamate Induce Ferroptosis without Affecting Mitochondrial Respiration in Rodent Brain Slice Cultures (2026) · cites this paper as Tang and Zucker, 1997
The Discussion describes mitochondrial calcium transport and says ATP production is unlikely to explain post-tetanic potentiation. Searches for glutamate uptake, 2-oxoglutarate, and glutamate-linked ATP synthesis found no passage in our copy addressing the attributed metabolic pathway. (LLM, conf=0.72)
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Cites
Mechanisms by which mitochondria transport calcium
Gunter, T. E., & Pfeiffer, D. R. (1990) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1990.258.5.c755 · Cited by 1,640 · see citing works
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Research works printed but not linked: 18
- [1]1. Aiuchi, T. ∙ Matsunaga, M. ∙ Nakaya, K. ... Effects of probes of membrane potential on metabolism in synaptosomes Biochem. Biophys. Acta. 1985; 843:20-24 Crossref Scopus (22) PubMed Google Scholar
- [2]2. Al-Baldawi, N.F. ∙ Moore, J.E. ∙ Abercrombie, R.F. Calcium accumulation by organelles within Myxicola axoplasm J. Physiol. 1993; 461:633-646 PubMed Google Scholar
- [3]3. Almers, W. ∙ Neher, E. The Ca signal from fura-2 loaded mast cells depends strongly on the method of dye-loading FEBS Lett. 1985; 192:13-18 Crossref Scopus (262) PubMed Google Scholar
- [4]4. Alnaes, E. ∙ Rahamimoff, R. On the role of mitochondria in transmitter release from motor nerve terminals J. Physiol. 1975; 248:285-306 PubMed Google Scholar
- [5]5. Atluri, P.P. ∙ Regehr, W.G. Determinants of the time course of facilitation at the granule cell to Purkinje cell synapse J. Neurosci. 1996; 16:5661-5771 PubMed Google Scholar
- [6]6. Blaustein, M.P. ∙ Ratzlaff, R.W. ∙ Schweitzer, E.S. Calcium buffering in presynaptic nerve terminals II. Kinetic properties of the nonmitochondrial Ca sequestration mechanism. J. Gen. Physiol. 1978; 72:43-66 Google Scholar
- [7]7. Brain, K.L. ∙ Bennett, M.R. Calcium in the nerve terminals of chick ciliary ganglia during facilitation, augmentation and potentiation J. Physiol. 1995; 489:637-648 PubMed Google Scholar
- [8]8. Brinley, Jr.F.J. Calcium buffering in squid axons Annu. Rev. Biophys. Bioeng. 1978; 7:363-392 Crossref Scopus (53) PubMed Google Scholar
- [9]9. Brorson, J.R. ∙ Bleakman, D. ∙ Gibbons, S.J. ... The properties of intracellular calcium stores in cultured rat cerebellar neurons J. Neurosci. 1991; 11:4024-4043 Crossref PubMed Google Scholar
- [10]10. Delaney, K.R. ∙ Tank, D.W. A quantitative measurement of the dependence of short-term synaptic enhancement on presynaptic residual calcium J. Neurosci. 1994; 14:5885-5902 PubMed Google Scholar
- [11]11. Delaney, K.R. ∙ Zucker, R.S. ∙ Tank, D.W. Calcium in motor nerve terminals associated with posttetanic potentiation J. Neurosci. 1989; 9:3558-3567 PubMed Google Scholar
- [12]12. Endo, M. ∙ Tanaka, M. ∙ Ogawa, Y. Calcium induced release of calcium from the sarcoplasmic reticulum of skinned skeletal muscle fibres Nature. 1970; 228:34-36 Crossref Scopus (592) PubMed Google Scholar
- [13]13. Fisher, S.A. ∙ Fischer, T.M. ∙ Carew, T.J. Multiple overlapping processes underlying short-term synaptic enhancement Trends Neurosci., in press. 1997; Google Scholar
- [14]14. Fossier, P. ∙ Baux, G. ∙ Tauc, L. Presynaptic mechanisms regulating Ca2+ concentration triggering acetylcholine release at an identified neuro-neuronal synapse of Aplysia Neuroscience. 1994; 63:405-414 Crossref Scopus (11) PubMed Google Scholar
- [15]15. Friel, D.D. ∙ Tsien, R.W. A caffeine- and ryanodine-sensitive Ca2+ store in bullfrog-sympathetic neurons modulates effects of Ca2+ entry on [Ca2+]i J. Physiol. 1992; 450:217-246 Crossref Scopus (248) PubMed Google Scholar
- [16]16. Friel, D.D. ∙ Tsien, R.W. An FCCP-sensitive Ca2+ store in bullfrog-sympathetic neurons and its participation in stimulus-evoked changes in [Ca2+]i J. Neurosci. 1994; 14:4007-4024 Crossref PubMed Google Scholar
- [17]17. Grohovaz, F. ∙ Bossi, M. ∙ Pezzati, R. ... High resolution ultrastructural mapping of total calciumelectron spectroscopic imaging / electron energy loss spectroscopy analysis of a physically/chemically processed nerve-muscle preparation Proc. Natl. Acad. Sci. USA. 1996; 93:4799-4803 Crossref Scopus (55) PubMed Google Scholar
- [18]18. Grynkiewicz, G. ∙ Poenie, M. ∙ Tsien, R.Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties J. Biol. Chem. 1985; 260:3440-3450
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- Authors
- Tang, Y.-G., & Zucker, R. S.
- Affiliations
- University of California, Berkeley (US)
- Corresponding author
- Zucker, Robert S.
- ORCID
- Zucker, Robert S.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1997-03
- Volume
- 18
- Issue
- 3
- Pages
- 483-491
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2034780161
- Cited by
- 441
- References
- 1
- Citations by year
- 2026: 10 · 2025: 4 · 2024: 10 · 2023: 17 · 2022: 13 · 2021: 11 · 2020: 15 · 2019: 9 · 2018: 11 · 2017: 13 · 2016: 24 · 2015: 14 · 2014: 15 · 2013: 13 · 2012: 16
- FWCI
- 7.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (NS 15114)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- post-tetanic potentiation, synaptic transmission, mitochondrial calcium, presynaptic calcium, crayfish neuromuscular junction, tetanic stimulation, tetraphenylphosphonium, CCCP, ruthenium red, endoplasmic reticulum calcium pump, thapsigargin, caffeine, calcium efflux
- MeSH terms
- Animals, Caffeine, Calcium, Carbonyl Cyanide m-Chlorophenyl Hydrazone, Astacoidea, Endoplasmic Reticulum, Mitochondria, Muscle Contraction, Synaptic Transmission, Neuromuscular Junction, Neuronal Plasticity, Onium Compounds, Organophosphorus Compounds, Oxidative Phosphorylation, Ruthenium Red, Uncoupling Agents, Thapsigargin
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